 |
SGC Experimental Information Sheet |
|
The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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|
| Target ID |
UBC60 |
| Description |
fused toes homolog; fused toes (mouse) homolog |
| Nucleotide accession |
NM_022476 |
| Protein accession |
NP_071921 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #2 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCAT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNH
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #3 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCC
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #4 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKS
|
| Vector |
p28a-thrombin-lic |
| Construct #5 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTG
TTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #6 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTG
TTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #7 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTG
TTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSP
WNPSVHDEAREKMLTQKKP
|
| Vector |
p28a-thrombin-lic |
| Construct #8 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTG
TTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAAGAACAGCACAATAAAAGTGT
TCATGTTGCTGGCCTGTCATGGGTAAAGCCT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSP
WNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #9 |
| Construct DNA sequence |
ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAGAAGACATTAACAGGGGACGT
GAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTGCCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTA
CATCTCCTGCCCCAGCAGCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTT
TGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAG
ATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAA
GATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTG
TTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAAGAACAGCACAATAAAAGTGT
TCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTACAGCCTTTCAGTAAAGAAGAGAAAACAGTGGCGACT
|
| Construct AA sequence |
MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLL
AEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDV
KRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSP
WNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #10 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #11 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCAT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNH
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #12 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CC
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #13 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKS
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #14 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTG
TGCACTGCTCGTTTGTTTGACCAACCT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKV
CTARLFDQP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #15 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTG
TGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKV
CTARLFDQPKIEDPYAISFSP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #16 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTG
TGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCATGGAATCCTTCTGTACA
TGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKV
CTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #17 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTG
TGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCATGGAATCCTTCTGTACA
TGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAAGAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGT
CATGGGTAAAGCCT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKV
CTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #18 |
| Construct DNA sequence |
GCACAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGT
TGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATAC
GGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGC
TTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAA
ATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCC
CCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTG
TGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCATGGAATCCTTCTGTACA
TGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAAGAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGT
CATGGGTAAAGCCTGGCTCAGTACAGCCTTTCAGTAAAGAAGAGAAAACAGTGGCGACT
|
| Construct AA sequence |
AQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPR
LVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKV
CTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #19 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #20 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCAT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNH
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #21 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCC
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #22 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAG
ATATTCAGCTTTTTAAAAGT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKS
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #23 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAG
ATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #24 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAG
ATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAA
GACCCCTATGCAATTAGCTTTTCTCCA
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIE
DPYAISFSP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #25 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAG
ATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAA
GACCCCTATGCAATTAGCTTTTCTCCATGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAA
GCCT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIE
DPYAISFSPWNPSVHDEAREKMLTQKKP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #26 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAG
ATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAA
GACCCCTATGCAATTAGCTTTTCTCCATGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAA
GCCTGAAGAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIE
DPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKP
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #27 |
| Construct DNA sequence |
CCCTTCTACCTGGAATACTCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCC
ATCTTATCGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTA
CAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTT
GATCCCACCTCAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATT
AATGTATGCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAG
ATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAA
GACCCCTATGCAATTAGCTTTTCTCCATGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAA
GCCTGAAGAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTACAGCCTTTCAGTA
AAGAAGAGAAAACAGTGGCGACT
|
| Construct AA sequence |
PFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLV
DPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIE
DPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-DE3
Expression outcome: Insoluble
|
| Construct #28 |
| Construct DNA sequence |
TCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATT
AATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATA
ACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAG
CTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGT
TTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAA
GTAAAGTTGTTGACAGTGTTAAGGTG
|
| Construct AA sequence |
SLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGE
LDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKV
|
| Vector |
p28a-thrombin-lic |
| Construct #29 |
| Construct DNA sequence |
TCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATT
AATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATA
ACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAG
CTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGT
TTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAA
GTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCT
|
| Construct AA sequence |
SLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGE
LDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQP
|
| Vector |
p28a-thrombin-lic |
| Construct #30 |
| Construct DNA sequence |
TCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATT
AATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATA
ACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAG
CTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGT
TTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAA
GTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGC
TTTTCTCCA
|
| Construct AA sequence |
SLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGE
LDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAIS
FSP
|
| Vector |
p28a-thrombin-lic |
| Construct #31 |
| Construct DNA sequence |
TCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATT
AATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATA
ACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAG
CTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGT
TTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAA
GTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGC
TTTTCTCCATGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCT
|
| Construct AA sequence |
SLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGE
LDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAIS
FSPWNPSVHDEAREKMLTQKKP
|
| Vector |
p28a-thrombin-lic |
| Construct #32 |
| Construct DNA sequence |
TCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATT
AATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATA
ACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAG
CTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGT
TTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAA
GTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGC
TTTTCTCCATGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAAGAACAGCACAA
TAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCT
|
| Construct AA sequence |
SLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGE
LDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAIS
FSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKP
|
| Vector |
p28a-thrombin-lic |
| Construct #33 |
| Construct DNA sequence |
TCTCTTCTTGCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTATCGCTCTGCATT
AATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGCGTATTTAAGTTTACAGTTTACATCCCTGATA
ACTATCCAGATGGTGACTGTCCACGCTTGGTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAG
CTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTATGCAAGGAGAGT
TTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTACTGTATGAAAAAGATATTCAGCTTTTTAAAA
GTAAAGTTGTTGACAGTGTTAAGGTGTGCACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGC
TTTTCTCCATGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAAGAACAGCACAA
TAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTACAGCCTTTCAGTAAAGAAGAGAAAACAGTGG
CGACT
|
| Construct AA sequence |
SLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGE
LDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAIS
FSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT
|
| Vector |
p28a-thrombin-lic |