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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
UBC58 |
| Description |
similar to 3110006E14Rik protein |
| Nucleotide accession |
XM_059689 |
| Protein accession |
XP_059689 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGATGCTGCGCCAGCTGTTAGCAGTTGCCGCAATGACTATGGAATGGCAGCAGCAACAACATCCAGGTGGTGCCGGCGA
TGCCAGTCCAGGACCAGGTAAAGGTAAAGGCAAACGGGCAGCGGAACTGGCACCGCGTGACAAAGCACCCGCGGCGGCCG
CTGCAGCCGCGGCCGCCGCAGCTGGTGCTGGTGGTCCGCGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCA
GTAGCGGCAGCGGCGGGTGGTTCTCTGGTCCCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCAT
GAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGA
ATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTG
AACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGT
GTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGC
GCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGA
AAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAG
|
| Construct AA sequence |
MMLRQLLAVAAMTMEWQQQQHPGGAGDASPGPGKGKGKRAAELAPRDKAPAAAAAAAAAAAGAGGPRERAAGARAGPGPA
VAAAAGGSLVPAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILL
NLTFPDNFPFSPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSR
KEAEATFKSLVKTHE
|
| Vector |
p28a-thrombin-lic |
| Construct #2 |
| Construct DNA sequence |
GATGCCAGTCCAGGACCAGGTAAAGGTAAAGGCAAACGGGCAGCGGAACTGGCACCGCGTGACAAAGCACCCGCGGCGGC
CGCTGCAGCCGCGGCCGCCGCAGCTGGTGCTGGTGGTCCGCGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGG
CAGTAGCGGCAGCGGCGGGTGGTTCTCTGGTCCCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTC
ATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTG
GAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCT
TGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTAT
GTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGAT
GCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCC
GAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAG
|
| Construct AA sequence |
DASPGPGKGKGKRAAELAPRDKAPAAAAAAAAAAAGAGGPRERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRL
MKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGY
VLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHE
|
| Vector |
p28a-thrombin-lic |
| Construct #3 |
| Construct DNA sequence |
GGCAAACGGGCAGCGGAACTGGCACCGCGTGACAAAGCACCCGCGGCGGCCGCTGCAGCCGCGGCCGCCGCAGCTGGTGC
TGGTGGTCCGCGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCAGTAGCGGCAGCGGCGGGTGGTTCTCTGG
TCCCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTA
AGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGA
TTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGT
TCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAG
CTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGG
TCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCT
TAGTCAAGACCCATGAG
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| Construct AA sequence |
GKRAAELAPRDKAPAAAAAAAAAAAGAGGPRERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRLMKELQDIARL
SDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGYVLDGGAICME
LLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHE
|
| Vector |
p28a-thrombin-lic |
| Construct #4 |
| Construct DNA sequence |
CCGCGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCAGTAGCGGCAGCGGCGGGTGGTTCTCTGGTCCCAGC
AGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATC
GTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTG
CTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCC
GCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCA
CCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGT
CGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAA
GACCCATGAG
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| Construct AA sequence |
PRERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSV
LWQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQG
RICRKAGKSKKSFSRKEAEATFKSLVKTHE
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| Vector |
p28a-thrombin-lic |
| Construct #5 |
| Construct DNA sequence |
GGGCCGGCAGTAGCGGCAGCGGCGGGTGGTTCTCTGGTCCCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCG
TCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTAT
TTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTT
ATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAA
CGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAG
CGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCG
TTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAG
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| Construct AA sequence |
GPAVAAAAGGSLVPAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEF
ILLNLTFPDNFPFSPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKS
FSRKEAEATFKSLVKTHE
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| Vector |
p28a-thrombin-lic |
| Construct #6 |
| Construct DNA sequence |
ACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACT
AGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAG
AAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTC
TCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATC
TGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGG
GCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAG
|
| Construct AA sequence |
TQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMRVL
SPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHE
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| Vector |
p28a-thrombin-lic |
| Construct #7 |
| Construct DNA sequence |
ATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTG
GAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCT
TGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTAT
GTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGAT
GCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCC
GAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAG
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| Construct AA sequence |
MKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGY
VLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHE
|
| Vector |
p28a-thrombin-lic |
| Construct #8 |
| Construct DNA sequence |
CCAGGACCAGGTAAAGGTAAAGGCAAACGGGCAGCGGAACTGGCACCGCGTGACAAAGCACCCGCGGCGGCCGCTGCAGC
CGCGGCCGCCGCAGCTGGTGCTGGTGGTCCGCGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCAGTAGCGG
CAGCGGCGGGTGGTTCTCTGGTCCCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAA
CTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAA
ACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGA
CCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGAT
GGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTT
CGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAG
CGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAGAAA
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| Construct AA sequence |
PGPGKGKGKRAAELAPRDKAPAAAAAAAAAAAGAGGPRERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRLMKE
LQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGYVLD
GGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHEK
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| Vector |
(p28a-lic-tev) |
| Construct #9 |
| Construct DNA sequence |
CCAGGACCAGGTAAAGGTAAAGGCAAACGGGCAGCGGAACTGGCACCGCGTGACAAAGCACCCGCGGCGGCCGCTGCAGC
CGCGGCCGCCGCAGCTGGTGCTGGTGGTCCGCGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCAGTAGCGG
CAGCGGCGGGTGGTTCTCTGGTCCCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAA
CTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAA
ACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGA
CCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGAT
GGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTT
CGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAG
CGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAGAAATACGGTTGG
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| Construct AA sequence |
PGPGKGKGKRAAELAPRDKAPAAAAAAAAAAAGAGGPRERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRLMKE
LQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGYVLD
GGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHEKYGW
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| Vector |
(p28a-lic-tev) |
| Construct #10 |
| Construct DNA sequence |
CGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCAGTAGCGGCAGCGGCGGGTGGTTCTCTGGTCCCAGCAGC
CCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTT
TCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTC
TGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCC
GTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCC
CGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGG
ATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGAC
CCATGAGAAA
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| Construct AA sequence |
RERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVL
WQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGR
ICRKAGKSKKSFSRKEAEATFKSLVKTHEK
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| Vector |
(p28a-lic-tev) |
| Construct #11 |
| Construct DNA sequence |
CGAGAACGTGCTGCAGGTGCGCGTGCTGGTCCGGGGCCGGCAGTAGCGGCAGCGGCGGGTGGTTCTCTGGTCCCAGCAGC
CCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTT
TCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTC
TGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCC
GTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCC
CGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGG
ATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGAC
CCATGAGAAATACGGTTGG
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| Construct AA sequence |
RERAAGARAGPGPAVAAAAGGSLVPAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVL
WQDMKETNTEFILLNLTFPDNFPFSPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGR
ICRKAGKSKKSFSRKEAEATFKSLVKTHEKYGW
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| Vector |
(p28a-lic-tev) |
| Construct #12 |
| Construct DNA sequence |
CCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAG
TGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATT
CTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTC
AGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCT
CCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTC
AGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTA
GTCAAGACCCATGAGAAA
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| Construct AA sequence |
PAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPF
SPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSL
VKTHEK
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| Vector |
(p28a-lic-tev) |
| Construct #13 |
| Construct DNA sequence |
CCAGCAGCCCGTCAACAGCATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAG
TGATCGTTTCATCAGCGTTGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATT
CTGTGCTCTGGCAGGATATGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTC
AGCCCGCCGTTTATGCGAGTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCT
CCTCACCCCGCGCGGTTGGTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTC
AGGGTCGGATTTGCCGCAAAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTA
GTCAAGACCCATGAGAAATACGGTTGG
|
| Construct AA sequence |
PAARQQHCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPF
SPPFMRVLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSL
VKTHEKYGW
|
| Vector |
(p28a-lic-tev) |
| Construct #14 |
| Construct DNA sequence |
CATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGT
TGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATA
TGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGA
GTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTG
GTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCA
AAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAGAAA
|
| Construct AA sequence |
HCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMR
VLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHEK
|
| Vector |
(p28a-lic-tev) |
| Construct #15 |
| Construct DNA sequence |
CATTGCACCCAAGTTCGTTCCCGTCGTCTCATGAAAGAACTCCAAGATATTGCGCGCTTAAGTGATCGTTTCATCAGCGT
TGAACTAGTTGATGAAAGCTTATTTGATTGGAATGTCAAACTACATCAGGTGGATAAAGATTCTGTGCTCTGGCAGGATA
TGAAAGAAACCAACACTGAATTTATTCTCTTGAACCTGACCTTTCCGGATAACTTTCCGTTCAGCCCGCCGTTTATGCGA
GTGCTCTCTCCAAGATTGGAAAACGGTTATGTGTTGGATGGTGGAGCCATTTGTATGGAGCTCCTCACCCCGCGCGGTTG
GTCATCTGCCTATACAGTCGAAGCGGTGATGCGCCAGTTCGCAGCAAGCCTGGTAAAGGGTCAGGGTCGGATTTGCCGCA
AAGCGGGCAAAAGCAAAAAATCGTTCTCCCGAAAAGAAGCGGAGGCGACCTTTAAGAGCTTAGTCAAGACCCATGAGAAA
TACGGTTGG
|
| Construct AA sequence |
HCTQVRSRRLMKELQDIARLSDRFISVELVDESLFDWNVKLHQVDKDSVLWQDMKETNTEFILLNLTFPDNFPFSPPFMR
VLSPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKSFSRKEAEATFKSLVKTHEK
YGW
|
| Vector |
(p28a-lic-tev) |