 |
SGC Experimental Information Sheet |
|
The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
|
|
| Target ID |
SULT6B1 |
| Description |
Human sulfotransferase SULT6B1 |
| Nucleotide accession |
NM_001032377.1 |
| Protein accession |
NP_001027549 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGTGCACCAGCGAAACCTTTCAGGCGCTGGATACCTTTGAAGCGCGTCATGATGACATTGTGCTGGCGAGCTATCCGAA
ATGCGGCAGCAACTGGATTCTGCATATTGTGAGCGAACTGATCTATGCGGTGAGCAAGAAAAAATATAAATATCCGGAAT
TTCCGGTGCTGGAATGCGGCGATAGCGAAAAATATCAGCGTATGAAAGGCTTTCCGAGCCCGCGTATTCTGGCGACCCAT
CTGCATTATGATAAACTGCCGGGCAGCATTTTTGAAAACAAAGCGAAAATTCTGGTGATTTTTCGTAACCCGAAAGATAC
CGCGGTGAGCTTTCTGCATTTTCATAACGATGTGCCGGATATTCCGAGCTATGGCAGCTGGGATGAATTCTTTCGTCAGT
TTATGAAAGGCCAGGTGAGCTGGGGCCGTTATTTTGATTTTGCGATTAACTGGAACAAACATCTGGATGGCGATAACGTG
AAATTCATTCTGTATGAAGATCTGAAAGAAAACCTGGCGGCCGGCATTAAACAGATTGCGGAATTTCTGGGCTTCTTTCT
GACCGGCGAACAGATTCAGACCATTAGCGTGCAGAGCACCTTTCAGGCGATGCGTGCGAAAAGCCAGGATACGCATGGCG
CGGTGGGCCCGTTTCTGTTTCGTAAAGGCAAAGTGGCGCTGTGGAAAAACCTGTTTAGCGAAATTCAGAACCAGGAAATG
GATGAAAAATTCAAAGAATGCCTGGCGGGCACCAGCCTGGGCGCGAAACTGAAATATGAAAGCTATTGCCAGGGCATTAG
CCCGTTTATGCGTAAAGGCATGCCGGGCGATTGGAAAAACTATTTTACCGTGGCGCAGAACGAAGAGTTTGATAAAGATT
ATCAGAAGAAAATGGCGGGCAGCACCCTGACCTTTCGTACCGAAATT
|
| Construct AA sequence |
MCTSETFQALDTFEARHDDIVLASYPKCGSNWILHIVSELIYAVSKKKYKYPEFPVLECGDSEKYQRMKGFPSPRILATH
LHYDKLPGSIFENKAKILVIFRNPKDTAVSFLHFHNDVPDIPSYGSWDEFFRQFMKGQVSWGRYFDFAINWNKHLDGDNV
KFILYEDLKENLAAGIKQIAEFLGFFLTGEQIQTISVQSTFQAMRAKSQDTHGAVGPFLFRKGKVALWKNLFSEIQNQEM
DEKFKECLAGTSLGAKLKYESYCQGISPFMRKGMPGDWKNYFTVAQNEEFDKDYQKKMAGSTLTFRTEI
|
| Vector |
p28-GST-lic |
| Construct #2 |
| Construct DNA sequence |
ACCAGCGAAACCTTTCAGGCGCTGGATACCTTTGAAGCGCGTCATGATGACATTGTGCTGGCGAGCTATCCGAAATGCGG
CAGCAACTGGATTCTGCATATTGTGAGCGAACTGATCTATGCGGTGAGCAAGAAAAAATATAAATATCCGGAATTTCCGG
TGCTGGAATGCGGCGATAGCGAAAAATATCAGCGTATGAAAGGCTTTCCGAGCCCGCGTATTCTGGCGACCCATCTGCAT
TATGATAAACTGCCGGGCAGCATTTTTGAAAACAAAGCGAAAATTCTGGTGATTTTTCGTAACCCGAAAGATACCGCGGT
GAGCTTTCTGCATTTTCATAACGATGTGCCGGATATTCCGAGCTATGGCAGCTGGGATGAATTCTTTCGTCAGTTTATGA
AAGGCCAGGTGAGCTGGGGCCGTTATTTTGATTTTGCGATTAACTGGAACAAACATCTGGATGGCGATAACGTGAAATTC
ATTCTGTATGAAGATCTGAAAGAAAACCTGGCGGCCGGCATTAAACAGATTGCGGAATTTCTGGGCTTCTTTCTGACCGG
CGAACAGATTCAGACCATTAGCGTGCAGAGCACCTTTCAGGCGATGCGTGCGAAAAGCCAGGATACGCATGGCGCGGTGG
GCCCGTTTCTGTTTCGTAAAGGCAAAGTGGCGCTGTGGAAAAACCTGTTTAGCGAAATTCAGAACCAGGAAATGGATGAA
AAATTCAAAGAATGCCTGGCGGGCACCAGCCTGGGCGCGAAACTGAAATATGAAAGCTATTGCCAGGGC
|
| Construct AA sequence |
TSETFQALDTFEARHDDIVLASYPKCGSNWILHIVSELIYAVSKKKYKYPEFPVLECGDSEKYQRMKGFPSPRILATHLH
YDKLPGSIFENKAKILVIFRNPKDTAVSFLHFHNDVPDIPSYGSWDEFFRQFMKGQVSWGRYFDFAINWNKHLDGDNVKF
ILYEDLKENLAAGIKQIAEFLGFFLTGEQIQTISVQSTFQAMRAKSQDTHGAVGPFLFRKGKVALWKNLFSEIQNQEMDE
KFKECLAGTSLGAKLKYESYCQG
|
| Vector |
p28a-thrombin-lic |
| Construct #3 |
| Construct DNA sequence |
ACCAGCGAAACCTTTCAGGCGCTGGATACCTTTGAAGCGCGTCATGATGACATTGTGCTGGCGAGCTATCCGAAATGCGG
CAGCAACTGGATTCTGCATATTGTGAGCGAACTGATCTATGCGGTGAGCAAGAAAAAATATAAATATCCGGAATTTCCGG
TGCTGGAATGCGGCGATAGCGAAAAATATCAGCGTATGAAAGGCTTTCCGAGCCCGCGTATTCTGGCGACCCATCTGCAT
TATGATAAACTGCCGGGCAGCATTTTTGAAAACAAAGCGAAAATTCTGGTGATTTTTCGTAACCCGAAAGATACCGCGGT
GAGCTTTCTGCATTTTCATAACGATGTGCCGGATATTCCGAGCTATGGCAGCTGGGATGAATTCTTTCGTCAGTTTATGA
AAGGCCAGGTGAGCTGGGGCCGTTATTTTGATTTTGCGATTAACTGGAACAAACATCTGGATGGCGATAACGTGAAATTC
ATTCTGTATGAAGATCTGAAAGAAAACCTGGCGGCCGGCATTAAACAGATTGCGGAATTTCTGGGCTTCTTTCTGACCGG
CGAACAGATTCAGACCATTAGCGTGCAGAGCACCTTTCAGGCGATGCGTGCGAAAAGCCAGGATACGCATGGCGCGGTGG
GCCCGTTTCTGTTTCGTAAAGGCAAAGTGGCGCTGTGGAAAAACCTGTTTAGCGAAATTCAGAACCAGGAAATGGATGAA
AAATTCAAAGAATGCCTGGCGGGCACCAGCCTGGGCGCGAAACTGAAATATGAAAGCTATAGCCAGGGC
|
| Construct AA sequence |
TSETFQALDTFEARHDDIVLASYPKCGSNWILHIVSELIYAVSKKKYKYPEFPVLECGDSEKYQRMKGFPSPRILATHLH
YDKLPGSIFENKAKILVIFRNPKDTAVSFLHFHNDVPDIPSYGSWDEFFRQFMKGQVSWGRYFDFAINWNKHLDGDNVKF
ILYEDLKENLAAGIKQIAEFLGFFLTGEQIQTISVQSTFQAMRAKSQDTHGAVGPFLFRKGKVALWKNLFSEIQNQEMDE
KFKECLAGTSLGAKLKYESYsQG
|
| Vector |
p28a-thrombin-lic |
| Construct #4 |
| Construct DNA sequence |
ACCAGCGAAACCTTTCAGGCGCTGGATACCTTTGAAGCGCGTCATGATGACATTGTGCTGGCGAGCTATCCGAAATGCGG
CAGCAACTGGATTCTGCATATTGTGAGCGAACTGATCTATGCGGTGAGCAAGAAAAAATATAAATATCCGGAATTTCCGG
TGCTGGAATGCGGCGATAGCGAAAAATATCAGCGTATGAAAGGCTTTCCGAGCCCGCGTATTCTGGCGACCCATCTGCAT
TATGATAAACTGCCGGGCAGCATTTTTGAAAACAAAGCGAAAATTCTGGTGATTTTTCGTAACCCGAAAGATACCGCGGT
GAGCTTTCTGCATTTTCATAACGATGTGCCGGATATTCCGAGCTATGGCAGCTGGGATGAATTCTTTCGTCAGTTTATGA
AAGGCCAGGTGAGCTGGGGCCGTTATTTTGATTTTGCGATTAACTGGAACAAACATCTGGATGGCGATAACGTGAAATTC
ATTCTGTATGAAGATCTGAAAGAAAACCTGGCGGCCGGCATTAAACAGATTGCGGAATTTCTGGGCTTCTTTCTGACCGG
CGAACAGATTCAGACCATTAGCGTGCAGAGCACCTTTCAGGCGATGCGTGCGAAAAGCCAGGATACGCATGGCGCGGTGG
GCCCGTTTCTGTTTCGTAAAGGCAAAGTGGCGCTGTGGAAAAACCTGTTTAGCGAAATTCAGAACCAGGAAATGGATGAA
AAATTCAAAGAATGCCTGGCGGGCACCAGCCTGGGCGCGAAACTGAAATATGAAAGCTATGCCCAGGGC
|
| Construct AA sequence |
TSETFQALDTFEARHDDIVLASYPKCGSNWILHIVSELIYAVSKKKYKYPEFPVLECGDSEKYQRMKGFPSPRILATHLH
YDKLPGSIFENKAKILVIFRNPKDTAVSFLHFHNDVPDIPSYGSWDEFFRQFMKGQVSWGRYFDFAINWNKHLDGDNVKF
ILYEDLKENLAAGIKQIAEFLGFFLTGEQIQTISVQSTFQAMRAKSQDTHGAVGPFLFRKGKVALWKNLFSEIQNQEMDE
KFKECLAGTSLGAKLKYESYaQG
|
| Vector |
p28a-thrombin-lic |
| Construct #5 |
| Construct DNA sequence |
ACCAGCGAAACCTTTCAGGCGCTGGATACCTTTGAAGCGCGTCATGATGACATTGTGCTGGCGAGCTATCCGAAATGCGG
CAGCAACTGGATTCTGCATATTGTGAGCGAACTGATCTATGCGGTGAGCAAGAAAAAATATAAATATCCGGAATTTCCGG
TGCTGGAATGCGGCGATAGCGAAAAATATCAGCGTATGAAAGGCTTTCCGAGCCCGCGTATTCTGGCGACCCATCTGCAT
TATGATAAACTGCCGGGCAGCATTTTTGAAAACAAAGCGAAAATTCTGGTGATTTTTCGTAACCCGAAAGATACCGCGGT
GAGCTTTCTGCATTTTCATAACGATGTGCCGGATATTCCGAGCTATGGCAGCTGGGATGAATTCTTTCGTCAGTTTATGA
AAGGCCAGGTGAGCTGGGGCCGTTATTTTGATTTTGCGATTAACTGGAACAAACATCTGGATGGCGATAACGTGAAATTC
ATTCTGTATGAAGATCTGAAAGAAAACCTGGCGGCCGGCATTAAACAGATTGCGGAATTTCTGGGCTTCTTTCTGACCGG
CGAACAGATTCAGACCATTAGCGTGCAGAGCACCTTTCAGGCGATGCGTGCGAAAAGCCAGGATACGCATGGCGCGGTGG
GCCCGTTTCTGTTTCGTAAAGGCAAAGTGGCGCTGTGGAAAAACCTGTTTAGCGAAATTCAGAACCAGGAAATGGATGAA
AAATTCAAAGAATGCCTGGCGGGCACCAGCCTGGGCGCGAAACTGAAATATGAAAGC
|
| Construct AA sequence |
TSETFQALDTFEARHDDIVLASYPKCGSNWILHIVSELIYAVSKKKYKYPEFPVLECGDSEKYQRMKGFPSPRILATHLH
YDKLPGSIFENKAKILVIFRNPKDTAVSFLHFHNDVPDIPSYGSWDEFFRQFMKGQVSWGRYFDFAINWNKHLDGDNVKF
ILYEDLKENLAAGIKQIAEFLGFFLTGEQIQTISVQSTFQAMRAKSQDTHGAVGPFLFRKGKVALWKNLFSEIQNQEMDE
KFKECLAGTSLGAKLKYES
|
| Vector |
p28a-thrombin-lic |