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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
PFI0525w |
| Description |
nucleotide binding protein, putative |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGAATGTATTCACAAAAAGAAGCTTCTTTTCCTTTTTTGTTGGATCCCTTGTAGGACTAAGTTTTAGTTATGTTCTAAC
TAAAAAGAAAAGTATAAACGAATTTATAAAATATGCAATAAAATATGTAACACATAAAAAAGGGAAAAAAAATAAAAATA
ATAATAATAATAATAATAACAGTATACCTGACGAATGCCCAGGTTTGGATAGCGAACATGCTGGGAAATCTAAAGCGTGT
GAAGGATGTCCAAATAGGAAAATATGTAATGATCCAGAATTAAAAAAAGAAAAAGAAAAAGAAAGAAATCAAATATTTAA
TGAGGTACAAGAAAATTTAAAAAATGTCAAGTTTAAAATTTTGATATTATCAGGTAAAGGAGGAGTAGGAAAATCAACAG
TGGCTGCACAATTAGCTTTTGCGTTATCATATTTAAATTATGAAGTTGGTTTATTAGATATCGATATTTGTGGTCCATCA
ATTCCGGTTTTAACTAAAACAATAGATCATGATGTAAATTATAGTATGAATGGATGGGTACCAATTTATAAAAATAATTT
ATCAATAATGTCTGTAGGTTATTTATTACCAAATTTTGATGACCCTGTTATATGGAGAGGTCCTAAAAAGAATGGATTAA
TAAAACAATTTTTATGTGATGTATACTGGAAAAATTTAGATTTTCTTATTATTGACACTCCACCAGGAACTAGTGATGAA
CATTTAACTATTTGTTCTTATTTAAAAGATAATTTAGATGGTTGCTTAATAGTAACTACTCCTCATATATTATCCATATG
TGACGTTAAAAAAGAAATCGAATTCTGTAAAAAAACAAATATACCAATATTAGGTATTGTTGAAAATATGTATCAACCAA
TTTTTGTATCAAATTATACAGTACAAAAAATGTGCTCTGATATGAATGTGCATTATGCCGGAAAGGTTACATTTCATCAG
AATCTAATCGAAGCTTGTCAACAAGGTGTTGGGTGTTGTGATATAGATCCATATAGTCCTTCATCGAAAGAATTATATCA
TCTTTGTAGTATTCTTATACAGCAAATTCAAAAGAGATACATGTTACTACAAATGGGTAGTAACAATGACAATAATAATT
TAGTACAAGAAAATAATCATCAAAACAAAGATAACACTAGAAACAGTGATAATCAAAAACTTACATATCAGGAAGAAGCA
TATGAAAATAATGTGAATAAAAATTATGATACATTAAGCAAATACAATGAACTAAATTATGACCATGCCAATTTATCAAA
ACTTCAAGTGCTAGATAAATTGTTAAGTGAAATAAATCCGTTTCTTGAGGAGAAAAAAGAATAA
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| Construct AA sequence |
MNVFTKRSFFSFFVGSLVGLSFSYVLTKKKSINEFIKYAIKYVTHKKGKKNKNNNNNNNNSIPDECPGLDSEHAGKSKAC
EGCPNRKICNDPELKKEKEKERNQIFNEVQENLKNVKFKILILSGKGGVGKSTVAAQLAFALSYLNYEVGLLDIDICGPS
IPVLTKTIDHDVNYSMNGWVPIYKNNLSIMSVGYLLPNFDDPVIWRGPKKNGLIKQFLCDVYWKNLDFLIIDTPPGTSDE
HLTICSYLKDNLDGCLIVTTPHILSICDVKKEIEFCKKTNIPILGIVENMYQPIFVSNYTVQKMCSDMNVHYAGKVTFHQ
NLIEACQQGVGCCDIDPYSPSSKELYHLCSILIQQIQKRYMLLQMGSNNDNNNLVQENNHQNKDNTRNSDNQKLTYQEEA
YENNVNKNYDTLSKYNELNYDHANLSKLQVLDKLLSEINPFLEEKKE
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| Vector |
p28a-thrombin-lic |
| Construct #2 |
| Construct DNA sequence |
AAAAAGAAAAGTATAAACGAATTTATAAAATATGCAATAAAATATGTAACACATAAAAAAGGGAAAAAAAATAAAAATAA
TAATAATAATAATAATAACAGTATACCTGACGAATGCCCAGGTTTGGATAGCGAACATGCTGGGAAATCTAAAGCGTGTG
AAGGATGTCCAAATAGGAAAATATGTAATGATCCAGAATTAAAAAAAGAAAAAGAAAAAGAAAGAAATCAAATATTTAAT
GAGGTACAAGAAAATTTAAAAAATGTCAAGTTTAAAATTTTGATATTATCAGGTAAAGGAGGAGTAGGAAAATCAACAGT
GGCTGCACAATTAGCTTTTGCGTTATCATATTTAAATTATGAAGTTGGTTTATTAGATATCGATATTTGTGGTCCATCAA
TTCCGGTTTTAACTAAAACAATAGATCATGATGTAAATTATAGTATGAATGGATGGGTACCAATTTATAAAAATAATTTA
TCAATAATGTCTGTAGGTTATTTATTACCAAATTTTGATGACCCTGTTATATGGAGAGGTCCTAAAAAGAATGGATTAAT
AAAACAATTTTTATGTGATGTATACTGGAAAAATTTAGATTTTCTTATTATTGACACTCCACCAGGAACTAGTGATGAAC
ATTTAACTATTTGTTCTTATTTAAAAGATAATTTAGATGGTTGCTTAATAGTAACTACTCCTCATATATTATCCATATGT
GACGTTAAAAAAGAAATCGAATTCTGTAAAAAAACAAATATACCAATATTAGGTATTGTTGAAAATATGTATCAACCAAT
TTTTGTATCAAATTATACAGTACAAAAAATGTGCTCTGATATGAATGTGCATTATGCCGGAAAGGTTACATTTCATCAGA
ATCTAATCGAAGCTTGTCAACAAGGTGTTGGGTGTTGTGATATAGATCCATATAGTCCTTCATCGAAAGAATTATATCAT
CTTTGTAGTATTCTTATACAGCAAATTCAAAAGAGATACATGTTACTACAAATGGGTAGTAACAATGACAATAATAATTT
AGTACAAGAAAATAATCATCAAAACAAAGATAACACTAGAAACAGTGATAATCAAAAACTTACATATCAGGAAGAAGCAT
ATGAAAATAATGTGAATAAAAATTATGATACATTAAGCAAATACAATGAACTAAATTATGACCATGCCAATTTATCAAAA
CTTCAAGTGCTAGATAAATTGTTAAGTGAAATAAATCCGTTTCTTGAGGAGAAAAAAGAATAA
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| Construct AA sequence |
KKKSINEFIKYAIKYVTHKKGKKNKNNNNNNNNSIPDECPGLDSEHAGKSKACEGCPNRKICNDPELKKEKEKERNQIFN
EVQENLKNVKFKILILSGKGGVGKSTVAAQLAFALSYLNYEVGLLDIDICGPSIPVLTKTIDHDVNYSMNGWVPIYKNNL
SIMSVGYLLPNFDDPVIWRGPKKNGLIKQFLCDVYWKNLDFLIIDTPPGTSDEHLTICSYLKDNLDGCLIVTTPHILSIC
DVKKEIEFCKKTNIPILGIVENMYQPIFVSNYTVQKMCSDMNVHYAGKVTFHQNLIEACQQGVGCCDIDPYSPSSKELYH
LCSILIQQIQKRYMLLQMGSNNDNNNLVQENNHQNKDNTRNSDNQKLTYQEEAYENNVNKNYDTLSKYNELNYDHANLSK
LQVLDKLLSEINPFLEEKKE
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| Vector |
p28a-thrombin-lic |