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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
PFI0385c |
| Description |
S1-P1nuclease, putative |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGCTCACTAAAAAAAAGTGTTGGAGACTAAAAGGTGGGATGTCTTTGGTATCTTTTTTTTGTTTTTTATCTTTTTTTTT
GTTTTTTTTCCTTTTTCATAATAATAATAATACCATATTATATGTGAATTGTTTTAATAATGAGGGGCATGAGGCGATAG
GTATGGTTGCGATGTCTGGTTTAAAGAATGAGCAATTATATGAGCTCAAGAAATTATTAAATGGTAAGGATTTAGTAGAT
ATTGGAAAATGGGGACATATAGTACATGATAAAATAAATGGAGCTAAAAATATGCATTTTAATTTACAAGAAAATGATTG
TAGAAATATAAATTTTGAATGTAAAGATACAAACGGTTTATGTTTAATAAATTCAATAAAATATTTTTATAATAAATTAT
TATCTACAAATCCTGATTCACAAAATAAAATATATAATAATATTGATAAAAAGGAGATATTAAAAAAATCCAAATTTATA
TATCCAAGAAATATTAATTTTACCGATTCAGATTCATTGAAATATTTAATATCCTTAATAAGTGATTTACATCAACCCTT
AAGAATAGGTTTTACCCATGATAATGGAGGTCAAGATATTAACATTACACATTTTAATATCGATGGAACCAAAGTTAAGA
CAAACCTCTTTCAATATATGGATAACGAAATTATAGATAAAATGATAAATAAATACCAATCATCATGGTATAGTGGATGG
ACACATATCAATCGTATTTTTGATGAACATAAAAAAGATGAAGAATTATTTGAACAACATGGAATAGATGTTATAGATAT
TTGGGCCAAACAAATTATAAGTGAATTCTGTTCAGAATTTTATCTTAATCATTATGTAACACATTTTATGATGAGTAAAG
GAGATCAATTACATTTTGATACTTCGAAAAATATTGATATATTTTATGATCTAGAGTTTGTATTAGAAAGATTGATACGT
TTTAATATATTACGAGCAGGATCTAGAATATCTATTATATTAAATTATATATTTTCAAAAAAAAAATTTTCGAATTTCAG
AAAAAAATCTGTTTTTGATAAGGGTAGAAAAAAAAAAAAAATTAAGAAAAATAAAAAAAAATAA
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| Construct AA sequence |
MLTKKKCWRLKGGMSLVSFFCFLSFFLFFFLFHNNNNTILYVNCFNNEGHEAIGMVAMSGLKNEQLYELKKLLNGKDLVD
IGKWGHIVHDKINGAKNMHFNLQENDCRNINFECKDTNGLCLINSIKYFYNKLLSTNPDSQNKIYNNIDKKEILKKSKFI
YPRNINFTDSDSLKYLISLISDLHQPLRIGFTHDNGGQDINITHFNIDGTKVKTNLFQYMDNEIIDKMINKYQSSWYSGW
THINRIFDEHKKDEELFEQHGIDVIDIWAKQIISEFCSEFYLNHYVTHFMMSKGDQLHFDTSKNIDIFYDLEFVLERLIR
FNILRAGSRISIILNYIFSKKKFSNFRKKSVFDKGRKKKKIKKNKKK
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| Vector |
p28a-thrombin-lic |