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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
PFF0625w |
| Description |
(protein coding) nucleolar GTP-binding protein 1, putative Location= chr6 531121..533181 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGAAAGAAAGCAATCTGTACCGCTTCAAAGATATAAAGCCTGTAGTTAGTGCTAAGGAACTTGTGGATGTGGTATTATC
AAAAACACAAAGGAAAACGCCCACAGAAATTCATAAAGGATTTAAGATAACAAGGATTAGGAATTTTTATATGAGGAAAG
TTAAAATATGTCAAGAATTATTTCGAGAAAAATTACAAAATATAATAAATGATTTCCCTAAATTAGATGATATCCATCCG
TTTTATTCGGATTTAGCAAATATATTATATGACAGAGATCATTATAAATTGTCATTAGGGCAATGTAGTTATGTTAGCAA
ATCATTAATAAGAATATGTCATGATTATATAAAACTTTTAAAATTTAGTTCATCTTTATATAAATGTAAAATGTTAAAAA
TAAGTGCACTAGGTAGAATGTGTAAATTAGTTAAGAAATTACAACCTAGTTTATTATATTTAGAAGAGGTTCGACAAAAT
TTATCAAGATTACCTTCTATTAATCCTCATAAAAAAACAATTATATTATCAGGAGCACCAAATGTAGGTAAATCATCTTT
TATGAATATTGTATCAAGAGCCAATGTAGATGTTCAATCCTATTCTTTTACTACAAAAAATTTATATGTAGGACATTTTG
ATCATAAATTAAACAAATATCAAATTATAGATACTCCAGGTTTATTAGATAGAGCATTCGAAAACAGAAACACTATTGAG
ATGACTACTATTACAGCACTTGCTCATATTAACGGAGTTATACTCTTTATTATAGATATAAGCGAACAATGTGGATTAAC
CATAAAAGAGCAAATTAATTTATTCTATTCTATTAAATCAGTCTTTTCCAATAAATCTATTGTTATCGGATTTAATAAAA
TCGACAAATGCAATATGGACAGCTTATCTATTGATAATAAATTATTAATAAAACAAATTCTTGATAATGTGAAAAATCCT
ATTAAATTCTCTTCATTTAGTACACTTACAGGAGTTGGAGTAGAACAAGCCAAAATAACTGCATGTGAGTTGCTAAAAAA
TGATCAAGCTGAATCTATCCTATTGGATCAAGAACAATTGCTAAACACCAAATTGGGTGAAACAAAAAACCACCTGAACA
GAACCCCATTTATACCTGAATCGGTCATACGAGAAAGGAAATTACGCCAAGAAAAAGCTCAATCCACAAACAACATGTTC
GATTCAACAAACGCAGATAGTATTACGTCCCCATGTGATCCAACGAATGCCACGGAAGGGGATCCCAATAAACAATATAC
CATGAGAGAATTAAAATTAAAAAAGAAAAATAAAAGCCGACAAAGTTATTTAAGTAACAGAACAGATGATAAAGTATTAC
AAATTGATCTTCAAAATGAAAATGGAGGAGCAGGTGTATACTCTGTAGATATTAGAAATAACTATGATATTAAAGAAGAA
TATAAATATGATGTAATACCAGAAATATATAACGGAAAAAATATTAGTGATTTTATAGATATGGATATAGAAAAAAAATT
ATTAGAATTAGAAAAAGAAGAAGAAGAATTATATGATAAGGATGCTAATATCGATCCTTTATGGTTTAAGACACAAAAAA
TTCTAGAAAAAATGAAATTAAGATTAAAGAGTGTTAGATTAGATGCAAAATTAAAAGATCAAAATGTTCAAATATATAAT
AAGAGAAAAAAAAATATTGAAGAAATAGAAAAGAAATTACTAGACCTGAAATTAGATAAAGAAAAAGTTATTAAAAGTAT
GACTGAAAAAGCAAGAAACAAAAATAAACAACCAGTAGTTAAAAAACAAAAAGTTAAAAAAGATATATCGAAGAAAAAAC
AAATTAAAGATAATATATATAATGATGAAAATAAATTATCCAAAATATTTTTAAGTACATCAACAGAATTACAAAGAAAG
AAAGCATATAAATTAAATAAAATGGCTTATAAAAAAATTACCAAAGGTACCAAAGGAGAGGCAGACAGATCAATACCGTC
CTTAAAGCCACGTCACTTATTTTCAGGAAAGCGTTCCATAGGAAAAACATCCCGTCGT
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| Construct AA sequence |
MKESNLYRFKDIKPVVSAKELVDVVLSKTQRKTPTEIHKGFKITRIRNFYMRKVKICQELFREKLQNIINDFPKLDDIHP
FYSDLANILYDRDHYKLSLGQCSYVSKSLIRICHDYIKLLKFSSSLYKCKMLKISALGRMCKLVKKLQPSLLYLEEVRQN
LSRLPSINPHKKTIILSGAPNVGKSSFMNIVSRANVDVQSYSFTTKNLYVGHFDHKLNKYQIIDTPGLLDRAFENRNTIE
MTTITALAHINGVILFIIDISEQCGLTIKEQINLFYSIKSVFSNKSIVIGFNKIDKCNMDSLSIDNKLLIKQILDNVKNP
IKFSSFSTLTGVGVEQAKITACELLKNDQAESILLDQEQLLNTKLGETKNHLNRTPFIPESVIRERKLRQEKAQSTNNMF
DSTNADSITSPCDPTNATEGDPNKQYTMRELKLKKKNKSRQSYLSNRTDDKVLQIDLQNENGGAGVYSVDIRNNYDIKEE
YKYDVIPEIYNGKNISDFIDMDIEKKLLELEKEEEELYDKDANIDPLWFKTQKILEKMKLRLKSVRLDAKLKDQNVQIYN
KRKKNIEEIEKKLLDLKLDKEKVIKSMTEKARNKNKQPVVKKQKVKKDISKKKQIKDNIYNDENKLSKIFLSTSTELQRK
KAYKLNKMAYKKITKGTKGEADRSIPSLKPRHLFSGKRSIGKTSRR
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| Vector |
p15-tev-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression outcome: Insoluble
|
| Construct #2 |
| Construct DNA sequence |
TTACCTTCTATTAATCCTCATAAAAAAACAATTATATTATCAGGAGCACCAAATGTAGGTAAATCATCTTTTATGAATAT
TGTATCAAGAGCCAATGTAGATGTTCAATCCTATTCTTTTACTACAAAAAATTTATATGTAGGACATTTTGATCATAAAT
TAAACAAATATCAAATTATAGATACTCCAGGTTTATTAGATAGAGCATTCGAAAACAGAAACACTATTGAGATGACTACT
ATTACAGCACTTGCTCATATTAACGGAGTTATACTCTTTATTATAGATATAAGCGAACAATGTGGATTAACCATAAAAGA
GCAAATTAATTTATTCTATTCTATTAAATCAGTCTTTTCCAATAAATCTATTGTTATCGGATTTAATAAAATCGACAAAT
GCAATATGGACAGCTTATCTATTGATAATAAATTATTAATAAAACAAATTCTTGATAATGTGAAAAATCCTATTAAATTC
TCTTCATTTAGTACACTTACAGGAGTTGGAGTAGAACAAGCCAAAATAACTGCATGTGAGTTGCTAAAAAATGATCAAGC
TGAATCTATCCTATTGGATCAAGAACAATTGCTAAACACCAAATTGGGTGAAACAAAAAAC
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| Construct AA sequence |
LPSINPHKKTIILSGAPNVGKSSFMNIVSRANVDVQSYSFTTKNLYVGHFDHKLNKYQIIDTPGLLDRAFENRNTIEMTT
ITALAHINGVILFIIDISEQCGLTIKEQINLFYSIKSVFSNKSIVIGFNKIDKCNMDSLSIDNKLLIKQILDNVKNPIKF
SSFSTLTGVGVEQAKITACELLKNDQAESILLDQEQLLNTKLGETKN
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| Vector |
p15-tev-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression outcome: Soluble##
Crystallization screens:
Red Wings screen,
SGC-I screen
No crystal |
| Construct #3 |
| Construct DNA sequence |
ATGAAAGAAAGCAATCTGTACCGCTTCAAAGATATAAAGCCTGTAGTTAGTGCTAAGGAACTTGTGGATGTGGTATTATC
AAAAACACAAAGGAAAACGCCCACAGAAATTCATAAAGGATTTAAGATAACAAGGATTAGGAATTTTTATATGAGGAAAG
TTAAAATATGTCAAGAATTATTTCGAGAAAAATTACAAAATATAATAAATGATTTCCCTAAATTAGATGATATCCATCCG
TTTTATTCGGATTTAGCAAATATATTATATGACAGAGATCATTATAAATTGTCATTAGGGCAATGTAGTTATGTTAGCAA
ATCATTAATAAGAATATGTCATGATTATATAAAACTTTTAAAATTTAGTTCATCTTTATATAAATGTAAAATGTTAAAAA
TAAGTGCACTAGGTAGAATGTGTAAATTAGTTAAGAAATTACAACCTAGTTTATTATATTTAGAAGAGGTTCGACAAAAT
TTATCAAGATTACCTTCTATTAATCCTCATAAAAAAACAATTATATTATCAGGAGCACCAAATGTAGGTAAATCATCTTT
TATGAATATTGTATCAAGAGCCAATGTAGATGTTCAATCCTATTCTTTTACTACAAAAAATTTATATGTAGGACATTTTG
ATCATAAATTAAACAAATATCAAATTATAGATACTCCAGGTTTATTAGATAGAGCATTCGAAAACAGAAACACTATTGAG
ATGACTACTATTACAGCACTTGCTCATATTAACGGAGTTATACTCTTTATTATAGATATAAGCGAACAATGTGGATTAAC
CATAAAAGAGCAAATTAATTTATTCTATTCTATTAAATCAGTCTTTTCCAATAAATCTATTGTTATCGGATTTAATAAAA
TCGACAAATGCAATATGGACAGCTTATCTATTGATAATAAATTATTAATAAAACAAATTCTTGATAATGTGAAAAATCCT
ATTAAATTCTCTTCATTTAGTACACTTACAGGAGTTGGAGTAGAACAAGCCAAAATAACTGCATGTGAGTTGCTAAAAAA
TGATCAAGCTGAATCTATCCTATTGGATCAAGAACAATTGCTAAACACCAAATTGGGTGAAACAAAAAACCACCTGAACA
GAACCCCATTTATACCTGAATCGGTCATACGAGAAAGGAAATTACGCCAAGAAAAAGCTCAATCCACAAACAACATGTTC
GATTCAACAAACGCAGATAGTATTACGTCCCCATGTGATCCAACGAATGCCACGGAAGGG
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| Construct AA sequence |
MKESNLYRFKDIKPVVSAKELVDVVLSKTQRKTPTEIHKGFKITRIRNFYMRKVKICQELFREKLQNIINDFPKLDDIHP
FYSDLANILYDRDHYKLSLGQCSYVSKSLIRICHDYIKLLKFSSSLYKCKMLKISALGRMCKLVKKLQPSLLYLEEVRQN
LSRLPSINPHKKTIILSGAPNVGKSSFMNIVSRANVDVQSYSFTTKNLYVGHFDHKLNKYQIIDTPGLLDRAFENRNTIE
MTTITALAHINGVILFIIDISEQCGLTIKEQINLFYSIKSVFSNKSIVIGFNKIDKCNMDSLSIDNKLLIKQILDNVKNP
IKFSSFSTLTGVGVEQAKITACELLKNDQAESILLDQEQLLNTKLGETKNHLNRTPFIPESVIRERKLRQEKAQSTNNMF
DSTNADSITSPCDPTNATEG
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| Vector |
p15-tev-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression outcome: Soluble##
Crystallization screens:
Red Wings screen,
SGC-I screen
No crystal |