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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
PF14_0477 |
| Description |
signal recognition particle 54 kDa protein, putative |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGGTGTTAACAGAACTAGGTACGCAAATTACGAACGCTTTTCGAAAACTTCAAACATCGACGTTAGCAGACGATGTTGT
AATAGAAGAATGTTTAAAAGAAATAATAAGGGCCTTAATATTATCTGATATAAATGTTAGTTATTTAAAAGATATTAAGA
GTAATATAAAGAATAATATAGAAAAGAATATAGATATATATGGAAACAATAAAAAGAAGCTAGTACAAAAATATGTGGTA
GAAGAATTAATAAAATTATTAGAAGGAAAAAAAGAGGGTTACAACCCGGTGAAAGGTAAAAGAAATGTTATATTATTTGT
AGGACTACAAGGAAGTGGGAAGACAACAACTTGTACAAAATATGCACATTATTATCAAAAGAAAGGTTTTAAGACTGCTT
TAGTATGTGCTGATACATTCAGAGCAGGTGCATTCGATCAATTAAAACAAAATGCAGCCAAAGTAAAAATTCCCTTTTAT
GGTAGTTATTCAGAAGTAGATCCCGTAAAAATAGCTACTGATGGTGTAAATGCTTTTTTAAAAGATAAATATGATTTAAT
AATTGTAGATAGTTCGGGGAGACATAAACAAGAAAATGAATTATTTGAAGAAATGATACAAGTAGAAAATTCTATACAAC
CAGAAGAAATTATTTTTGTTATTGATAGCCATATTGGCCAAAGTTGTCATGATCAAGCTATGGCATTTAAAAATTCTGTA
TCCTTAGGAAGTATAATTATAACAAAGATAGATGGGCATGCTAAAGGTGGTGGAGCTTTATCTGCCGTAGCTGCTACCGG
ATGTCCTATAACATTTATAGGAACAGGGGAACATGTTAATGATTTTGAAAAATTCGAAGCAAAATCTTTTGTATCTAGAT
TATTAGGCTTAGGAGATATTAGTGGACTTGTATCTACTATTAAAGAAGTTATAGATATAGATAAACAACCTGAATTAATG
AATAGATTATCTAAAGGAAAATTTGTTTTGAGAGATATGTACGATCAATTTCAAAATGTATTTAAAATGGGTAGTCTTAG
TAAAGTTATGTCTATGATACCAGGTTTTGGTAATAATTTAATTAGTAAAGGTACGGAAAAAGAAGGAATCGATAAAATAA
AGAAATTTATGGTAATTATGGATTCTATGACAAATGAAGAATTAGATTGTATTAAACCACTTAATGATAGTAGATGCTTA
AGAATTGTAAAGGGGTCCGGTACTCGCCTTCAAGATATTAAAGAACTTTTAGAACAATTCAAATTCTTACAAAAAATGGT
TGTAAAAATGGGAAAATTAGGATTAAGAGAAAATAACATAAGTAATTTAATGAGAAACCAAAAACAATTCATGAGCAAAA
TGAATAATATAATGGATCCAAATATGTTAAAACAATTAGGAGGAGCTAATAATATGGTCAACATTTTAAAAGAATTTACA
AAAATGGATGACTTGGGGGTATCAATGGCTAACATGATGAAGCAAATGGGTCTTAAAAAA
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| Construct AA sequence |
MVLTELGTQITNAFRKLQTSTLADDVVIEECLKEIIRALILSDINVSYLKDIKSNIKNNIEKNIDIYGNNKKKLVQKYVV
EELIKLLEGKKEGYNPVKGKRNVILFVGLQGSGKTTTCTKYAHYYQKKGFKTALVCADTFRAGAFDQLKQNAAKVKIPFY
GSYSEVDPVKIATDGVNAFLKDKYDLIIVDSSGRHKQENELFEEMIQVENSIQPEEIIFVIDSHIGQSCHDQAMAFKNSV
SLGSIIITKIDGHAKGGGALSAVAATGCPITFIGTGEHVNDFEKFEAKSFVSRLLGLGDISGLVSTIKEVIDIDKQPELM
NRLSKGKFVLRDMYDQFQNVFKMGSLSKVMSMIPGFGNNLISKGTEKEGIDKIKKFMVIMDSMTNEELDCIKPLNDSRCL
RIVKGSGTRLQDIKELLEQFKFLQKMVVKMGKLGLRENNISNLMRNQKQFMSKMNNIMDPNMLKQLGGANNMVNILKEFT
KMDDLGVSMANMMKQMGLKK
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| Vector |
p15-tev-lic |
| Construct #2 |
| Construct DNA sequence |
AAATTTGTTTTGAGAGATATGTACGATCAATTTCAAAATGTATTTAAAATGGGTAGTCTTAGTAAAGTTATGTCTATGAT
ACCAGGTTTTGGTAATAATTTAATTAGTAAAGGTACGGAAAAAGAAGGAATCGATAAAATAAAGAAATTTATGGTAATTA
TGGATTCTATGACAAATGAAGAATTAGATTGTATTAAACCACTTAATGATAGTAGATGCTTAAGAATTGTAAAGGGGTCC
GGTACTCGCCTTCAAGATATTAAAGAACTTTTAGAACAATTCAAATTCTTACAAAAAATGGTTGTAAAAATGGGAAAATT
A
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| Construct AA sequence |
KFVLRDMYDQFQNVFKMGSLSKVMSMIPGFGNNLISKGTEKEGIDKIKKFMVIMDSMTNEELDCIKPLNDSRCLRIVKGS
GTRLQDIKELLEQFKFLQKMVVKMGKL
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| Vector |
p15-tev-lic |
| Construct #3 |
| Construct DNA sequence |
ATGGTGTTAACAGAACTAGGTACGCAAATTACGAACGCTTTTCGAAAACTTCAAACATCGACGTTAGCAGACGATGTTGT
AATAGAAGAATGTTTAAAAGAAATAATAAGGGCCTTAATATTATCTGATATAAATGTTAGTTATTTAAAAGATATTAAGA
GTAATATAAAGAATAATATAGAAAAGAATATAGATATATATGGAAACAATAAAAAGAAGCTAGTACAAAAATATGTGGTA
GAAGAATTAATAAAATTATTAGAAGGAAAAAAAGAGGGTTACAACCCGGTGAAAGGTAAAAGAAATGTTATATTATTTGT
AGGACTACAAGGAAGTGGGAAGACAACAACTTGTACAAAATATGCACATTATTATCAAAAGAAAGGTTTTAAGACTGCTT
TAGTATGTGCTGATACATTCAGAGCAGGTGCATTCGATCAATTAAAACAAAATGCAGCCAAAGTAAAAATTCCCTTTTAT
GGTAGTTATTCAGAAGTAGATCCCGTAAAAATAGCTACTGATGGTGTAAATGCTTTTTTAAAAGATAAATATGATTTAAT
AATTGTAGATAGTTCGGGGAGACATAAACAAGAAAATGAATTATTTGAAGAAATGATACAAGTAGAAAATTCTATACAAC
CAGAAGAAATTATTTTTGTTATTGATAGCCATATTGGCCAAAGTTGTCATGATCAAGCTATGGCATTTAAAAATTCTGTA
TCCTTAGGAAGTATAATTATAACAAAGATAGATGGGCATGCTAAAGGTGGTGGAGCTTTATCTGCCGTAGCTGCTACCGG
ATGTCCTATAACATTTATAGGAACAGGGGAACATGTTAATGATTTTGAAAAATTCGAAGCAAAATCTTTTGTATCTAGAT
TATTAGGCTTA
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| Construct AA sequence |
MVLTELGTQITNAFRKLQTSTLADDVVIEECLKEIIRALILSDINVSYLKDIKSNIKNNIEKNIDIYGNNKKKLVQKYVV
EELIKLLEGKKEGYNPVKGKRNVILFVGLQGSGKTTTCTKYAHYYQKKGFKTALVCADTFRAGAFDQLKQNAAKVKIPFY
GSYSEVDPVKIATDGVNAFLKDKYDLIIVDSSGRHKQENELFEEMIQVENSIQPEEIIFVIDSHIGQSCHDQAMAFKNSV
SLGSIIITKIDGHAKGGGALSAVAATGCPITFIGTGEHVNDFEKFEAKSFVSRLLGL
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| Vector |
p15-tev-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression protocol: lexsop1.1
Expression outcome: Soluble
Crystallization screens:
Red Wings screen,
SGC-I screen
No crystal |