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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
NR4A3 |
| Description |
nuclear receptor subfamily 4, group A, member 3 |
| Nucleotide accession |
NM_006981 |
| Protein accession |
NP_008912 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
GGTCGTCTGCCTTCCAAACCAAAGAGCCCATTACAACAGGAACCTTCTCAGCCCTCTCCACCTTCTCCTCCAATCTGCAT
GATGAATGCTCTTGTCCGAGCTTTAACAGACTCAACACCCAGAGATCTTGATTATTCCAGATACTGTCCCACTGACCAGG
CTGCTGCAGGCACAGATGCTGAGCATGTGCAACAATTCTACAACCTCCTGACAGCCTCCATTGATGTATCCAGAAGCTGG
GCAGAAAAGATTCCGGGATTTACTGATCTCCCCAAAGAAGATCAGACATTACTTATTGAATCAGCCTTTTTGGAGCTGTT
TGTCCTCAGACTTTCCATCAGGTCAAACACTGCTGAAGATAAGTTTGTGTTCTGCAATGGACTTGTCCTGCATCGACTTC
AGTGCCTTCGTGGATTTGGGGAGTGGCTCGACTCTATTAAAGACTTTTCCTTAAATTTGCAGAGCCTGAACCTTGATATC
CAAGCCTTAGCCTGCCTGTCAGCACTGAGCATGATCACAGAAAGACATGGGTTAAAAGAACCAAAGAGAGTCGAAGAGCT
ATGCAACAAGATCACAAGCAGTTTAAAAGACCACCAGAGTAAGGGACAGGCTCTGGAGCCCACCGAGTCCAAGGTCCTGG
GTGCCCTGGTAGAACTGAGGAAGATCTGCACCCTGGGCCTCCAGCGCATCTTCTACCTGAAGCTGGAAGACTTGGTGTCT
CCACCTTCCATCATTGACAAGCTCTTCCTGGACACCCTACCTTTC
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| Construct AA sequence |
GRLPSKPKSPLQQEPSQPSPPSPPICMMNALVRALTDSTPRDLDYSRYCPTDQAAAGTDAEHVQQFYNLLTASIDVSRSW
AEKIPGFTDLPKEDQTLLIESAFLELFVLRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKDFSLNLQSLNLDI
QALACLSALSMITERHGLKEPKRVEELCNKITSSLKDHQSKGQALEPTESKVLGALVELRKICTLGLQRIFYLKLEDLVS
PPSIIDKLFLDTLPF
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| Vector |
p28a-thrombin-lic |
| Construct #2 |
| Construct DNA sequence |
TCTCCACCTTCTCCTCCAATCTGCATGATGAATGCTCTTGTCCGAGCTTTAACAGACTCAACACCCAGAGATCTTGATTA
TTCCAGATACTGTCCCACTGACCAGGCTGCTGCAGGCACAGATGCTGAGCATGTGCAACAATTCTACAACCTCCTGACAG
CCTCCATTGATGTATCCAGAAGCTGGGCAGAAAAGATTCCGGGATTTACTGATCTCCCCAAAGAAGATCAGACATTACTT
ATTGAATCAGCCTTTTTGGAGCTGTTTGTCCTCAGACTTTCCATCAGGTCAAACACTGCTGAAGATAAGTTTGTGTTCTG
CAATGGACTTGTCCTGCATCGACTTCAGTGCCTTCGTGGATTTGGGGAGTGGCTCGACTCTATTAAAGACTTTTCCTTAA
ATTTGCAGAGCCTGAACCTTGATATCCAAGCCTTAGCCTGCCTGTCAGCACTGAGCATGATCACAGAAAGACATGGGTTA
AAAGAACCAAAGAGAGTCGAAGAGCTATGCAACAAGATCACAAGCAGTTTAAAAGACCACCAGAGTAAGGGACAGGCTCT
GGAGCCCACCGAGTCCAAGGTCCTGGGTGCCCTGGTAGAACTGAGGAAGATCTGCACCCTGGGCCTCCAGCGCATCTTCT
ACCTGAAGCTGGAAGACTTGGTGTCTCCACCTTCCATCATTGACAAGCTCTTCCTGGACACCCTACCTTTC
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| Construct AA sequence |
SPPSPPICMMNALVRALTDSTPRDLDYSRYCPTDQAAAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGFTDLPKEDQTLL
IESAFLELFVLRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKDFSLNLQSLNLDIQALACLSALSMITERHGL
KEPKRVEELCNKITSSLKDHQSKGQALEPTESKVLGALVELRKICTLGLQRIFYLKLEDLVSPPSIIDKLFLDTLPF
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| Vector |
p28a-thrombin-lic |
| Construct #3 |
| Construct DNA sequence |
AATGCTCTTGTCCGAGCTTTAACAGACTCAACACCCAGAGATCTTGATTATTCCAGATACTGTCCCACTGACCAGGCTGC
TGCAGGCACAGATGCTGAGCATGTGCAACAATTCTACAACCTCCTGACAGCCTCCATTGATGTATCCAGAAGCTGGGCAG
AAAAGATTCCGGGATTTACTGATCTCCCCAAAGAAGATCAGACATTACTTATTGAATCAGCCTTTTTGGAGCTGTTTGTC
CTCAGACTTTCCATCAGGTCAAACACTGCTGAAGATAAGTTTGTGTTCTGCAATGGACTTGTCCTGCATCGACTTCAGTG
CCTTCGTGGATTTGGGGAGTGGCTCGACTCTATTAAAGACTTTTCCTTAAATTTGCAGAGCCTGAACCTTGATATCCAAG
CCTTAGCCTGCCTGTCAGCACTGAGCATGATCACAGAAAGACATGGGTTAAAAGAACCAAAGAGAGTCGAAGAGCTATGC
AACAAGATCACAAGCAGTTTAAAAGACCACCAGAGTAAGGGACAGGCTCTGGAGCCCACCGAGTCCAAGGTCCTGGGTGC
CCTGGTAGAACTGAGGAAGATCTGCACCCTGGGCCTCCAGCGCATCTTCTACCTGAAGCTGGAAGACTTGGTGTCTCCAC
CTTCCATCATTGACAAGCTCTTCCTGGACACCCTACCTTTC
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| Construct AA sequence |
NALVRALTDSTPRDLDYSRYCPTDQAAAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGFTDLPKEDQTLLIESAFLELFV
LRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKDFSLNLQSLNLDIQALACLSALSMITERHGLKEPKRVEELC
NKITSSLKDHQSKGQALEPTESKVLGALVELRKICTLGLQRIFYLKLEDLVSPPSIIDKLFLDTLPF
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| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression outcome: Soluble
Crystallization screens:
Red Wings screen,
SGC-I screen
No crystal |