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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
NR2F1 |
| Description |
nuclear receptor subfamily 2, group F, member 1 |
| Nucleotide accession |
NM_005654 |
| Protein accession |
NP_005645 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGGTAGTTAGCAGCTGGCGAGATCCGCAGGACGACGTGGCCGGGGGCAACCCCGGCGGCCCCAACCCCGCAGCGCAGGC
GGCCCGCGGCGGCGGCGGCGGCGCCGGCGAGCAGCAGCAGCAGGCGGGCTCGGGCGCGCCGCACACGCCGCAGACCCCGG
GCCAGCCCGGAGCGCCCGCCACCCCCGGCACGGCGGGGGACAAGGGCCAGGGCCCGCCCGGTTCGGGCCAGAGCCAGCAG
CACATCGAGTGCGTGGTGTGCGGGGACAAGTCGAGCGGCAAGCACTACGGCCAATTCACCTGCGAGGGCTGCAAAAGTTT
CTTCAAGAGGAGCGTCCGCAGGAACTTAACTTACACATGCCGTGCCAACAGGAACTGTCCCATCGACCAGCACCACCGCA
ACCAGTGCCAATACTGCCGCCTCAAGAAGTGCCTCAAAGTGGGCATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCT
CCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACAT
CTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCA
TCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGAT
CTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTAT
GCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCA
TGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTC
AAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCA
GTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCT
CGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTC
ATCCGCGATATGTTACTGTCTGGGAGCAGCTTCAACTGGCCTTACATGTCCATCCAGTGCTCC
|
| Construct AA sequence |
MVVSSWRDPQDDVAGGNPGGPNPAAQAARGGGGGAGEQQQQAGSGAPHTPQTPGQPGAPATPGTAGDKGQGPPGSGQSQQ
HIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMP
PTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPD
LQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCL
KAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETL
IRDMLLSGSSFNWPYMSIQCS
|
| Vector |
p11 |
| Construct #2 |
| Construct DNA sequence |
ATGGTAGTTAGCAGCTGGCGAGATCCGCAGGACGACGTGGCCGGGGGCAACCCCGGCGGCCCCAACCCCGCAGCGCAGGC
GGCCCGCGGCGGCGGCGGCGGCGCCGGCGAGCAGCAGCAGCAGGCGGGCTCGGGCGCGCCGCACACGCCGCAGACCCCGG
GCCAGCCCGGAGCGCCCGCCACCCCCGGCACGGCGGGGGACAAGGGCCAGGGCCCGCCCGGTTCGGGCCAGAGCCAGCAG
CACATCGAGTGCGTGGTGTGCGGGGACAAGTCGAGCGGCAAGCACTACGGCCAATTCACCTGCGAGGGCTGCAAAAGTTT
CTTCAAGAGGAGCGTCCGCAGGAACTTAACTTACACATGCCGTGCCAACAGGAACTGTCCCATCGACCAGCACCACCGCA
ACCAGTGCCAATACTGCCGCCTCAAGAAGTGCCTCAAAGTGGGCATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCT
CCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACAT
CTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCA
TCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGAT
CTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTAT
GCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCA
TGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTC
AAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCA
GTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCT
CGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTC
ATCCGCGATATGTTACTGTCTGGGAGCAGC
|
| Construct AA sequence |
MVVSSWRDPQDDVAGGNPGGPNPAAQAARGGGGGAGEQQQQAGSGAPHTPQTPGQPGAPATPGTAGDKGQGPPGSGQSQQ
HIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMP
PTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPD
LQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCL
KAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETL
IRDMLLSGSS
|
| Vector |
p11 |
| Construct #3 |
| Construct DNA sequence |
ATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCTCCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGA
CCCCCTCAACGGCCACTGCTACCTGTCCGGCTACATCTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACG
GCAGCCAGTGCATGCAGCCCAACAACATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCC
GTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAG
CGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTATGCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATG
CCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCATGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAG
GCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGA
TGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCAGTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGC
CCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTC
GTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTCATCCGCGATATGTTACTGTCTGGGAGCAGC
|
| Construct AA sequence |
MRREAVQRGRMPPTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSA
VEWARNIPFFPDLQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLK
ALHVDSAEYSCLKAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFF
VRLVGKTPIETLIRDMLLSGSS
|
| Vector |
p11 |
| Construct #4 |
| Construct DNA sequence |
ATGCCTCCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGG
CTACATCTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTA
TGGGCATCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTC
CCGGATCTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTG
CTCTATGCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGG
CCTTCATGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGC
TGCCTCAAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAA
GTCGCAGTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGAC
TGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAA
ACTCTCATCCGCGATATGTTACTGTCTGGGAGCAGC
|
| Construct AA sequence |
MPPTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFF
PDLQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYS
CLKAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIE
TLIRDMLLSGSS
|
| Vector |
p11 |
| Construct #5 |
| Construct DNA sequence |
GCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACATCTCGCTGCTGCTGCGCGCCGAGCCCTA
CCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGC
GCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTG
CTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTATGCCGCTGCACGTGGCGCCGTTGCTGGC
CGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCATGGACCACATCCGCATCTTCCAGGAGC
AGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGAC
GCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCAGTGCGCACTGGAGGAGTACGTGAGGAG
CCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCA
TCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTCATCCGCGATATGTTACTGTCTGGGAGC
AGC
|
| Construct AA sequence |
ALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPDLQITDQVSL
LRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSD
ACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGS
S
|
| Vector |
p11 |
| Construct #6 |
| Construct DNA sequence |
GCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACATCTCGCTGCTGCTGCGCGCCGAGCCCTA
CCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGC
GCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTG
CTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTATGCCGCTGCACGTGGCGCCGTTGCTGGC
CGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCATGGACCACATCCGCATCTTCCAGGAGC
AGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGAC
GCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCAGTGCGCACTGGAGGAGTACGTGAGGAG
CCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCA
TCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTCATCCGCGATATGTTACTGTCTGGGAGC
AGCTTCAACTGGCCTTACATGTCCATCCAGTGCTCC
|
| Construct AA sequence |
ALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPDLQITDQVSL
LRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSD
ACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGS
SFNWPYMSIQCS
|
| Vector |
p11 |
| Construct #7 |
| Construct DNA sequence |
ATGGTAGTTAGCAGCTGGCGAGATCCGCAGGACGACGTGGCCGGGGGCAACCCCGGCGGCCCCAACCCCGCAGCGCAGGC
GGCCCGCGGCGGCGGCGGCGGCGCCGGCGAGCAGCAGCAGCAGGCGGGCTCGGGCGCGCCGCACACGCCGCAGACCCCGG
GCCAGCCCGGAGCGCCCGCCACCCCCGGCACGGCGGGGGACAAGGGCCAGGGCCCGCCCGGTTCGGGCCAGAGCCAGCAG
CACATCGAGTGCGTGGTGTGCGGGGACAAGTCGAGCGGCAAGCACTACGGCCAATTCACCTGCGAGGGCTGCAAAAGTTT
CTTCAAGAGGAGCGTCCGCAGGAACTTAACTTACACATGCCGTGCCAACAGGAACTGTCCCATCGACCAGCACCACCGCA
ACCAGTGCCAATACTGCCGCCTCAAGAAGTGCCTCAAAGTGGGCATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCT
CCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACAT
CTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCA
TCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGAT
CTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTAT
GCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCA
TGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTC
AAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCA
GTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCT
CGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTC
ATCCGCGATATGTTACTGTCTGGGAGCAGCTTCAACTGGCCTTACATGTCCATCCAGTGCTCC
|
| Construct AA sequence |
MVVSSWRDPQDDVAGGNPGGPNPAAQAARGGGGGAGEQQQQAGSGAPHTPQTPGQPGAPATPGTAGDKGQGPPGSGQSQQ
HIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMP
PTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPD
LQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCL
KAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETL
IRDMLLSGSSFNWPYMSIQCS
|
| Vector |
p28a-thrombin-lic |
| Construct #8 |
| Construct DNA sequence |
ATGGTAGTTAGCAGCTGGCGAGATCCGCAGGACGACGTGGCCGGGGGCAACCCCGGCGGCCCCAACCCCGCAGCGCAGGC
GGCCCGCGGCGGCGGCGGCGGCGCCGGCGAGCAGCAGCAGCAGGCGGGCTCGGGCGCGCCGCACACGCCGCAGACCCCGG
GCCAGCCCGGAGCGCCCGCCACCCCCGGCACGGCGGGGGACAAGGGCCAGGGCCCGCCCGGTTCGGGCCAGAGCCAGCAG
CACATCGAGTGCGTGGTGTGCGGGGACAAGTCGAGCGGCAAGCACTACGGCCAATTCACCTGCGAGGGCTGCAAAAGTTT
CTTCAAGAGGAGCGTCCGCAGGAACTTAACTTACACATGCCGTGCCAACAGGAACTGTCCCATCGACCAGCACCACCGCA
ACCAGTGCCAATACTGCCGCCTCAAGAAGTGCCTCAAAGTGGGCATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCT
CCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACAT
CTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCA
TCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGAT
CTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTAT
GCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCA
TGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTC
AAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCA
GTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCT
CGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTC
ATCCGCGATATGTTACTGTCTGGGAGCAGC
|
| Construct AA sequence |
MVVSSWRDPQDDVAGGNPGGPNPAAQAARGGGGGAGEQQQQAGSGAPHTPQTPGQPGAPATPGTAGDKGQGPPGSGQSQQ
HIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMP
PTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPD
LQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCL
KAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETL
IRDMLLSGSS
|
| Vector |
p28a-thrombin-lic |
| Construct #9 |
| Construct DNA sequence |
ATGAGGCGGGAAGCGGTTCAGCGAGGAAGAATGCCTCCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGA
CCCCCTCAACGGCCACTGCTACCTGTCCGGCTACATCTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACG
GCAGCCAGTGCATGCAGCCCAACAACATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCC
GTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAG
CGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTATGCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATG
CCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCATGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAG
GCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGA
TGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCAGTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGC
CCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTC
GTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTCATCCGCGATATGTTACTGTCTGGGAGCAGC
|
| Construct AA sequence |
MRREAVQRGRMPPTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSA
VEWARNIPFFPDLQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLK
ALHVDSAEYSCLKAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFF
VRLVGKTPIETLIRDMLLSGSS
|
| Vector |
p28a-thrombin-lic |
| Construct #10 |
| Construct DNA sequence |
ATGCCTCCAACCCAGCCCAATCCAGGCCAGTACGCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGG
CTACATCTCGCTGCTGCTGCGCGCCGAGCCCTACCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTA
TGGGCATCGAGAACATCTGCGAGCTGGCCGCGCGCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTC
CCGGATCTGCAGATCACCGACCAGGTGTCCCTGCTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTG
CTCTATGCCGCTGCACGTGGCGCCGTTGCTGGCCGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGG
CCTTCATGGACCACATCCGCATCTTCCAGGAGCAGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGC
TGCCTCAAAGCCATCGTGCTGTTCACGTCAGACGCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAA
GTCGCAGTGCGCACTGGAGGAGTACGTGAGGAGCCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGAC
TGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCATCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAA
ACTCTCATCCGCGATATGTTACTGTCTGGGAGCAGC
|
| Construct AA sequence |
MPPTQPNPGQYALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFF
PDLQITDQVSLLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYS
CLKAIVLFTSDACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIE
TLIRDMLLSGSS
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression outcome: Soluble
Crystallization screens:
Red Wings screen,
SGC-I screen
No crystal |
| Construct #11 |
| Construct DNA sequence |
GCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACATCTCGCTGCTGCTGCGCGCCGAGCCCTA
CCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGC
GCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTG
CTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTATGCCGCTGCACGTGGCGCCGTTGCTGGC
CGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCATGGACCACATCCGCATCTTCCAGGAGC
AGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGAC
GCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCAGTGCGCACTGGAGGAGTACGTGAGGAG
CCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCA
TCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTCATCCGCGATATGTTACTGTCTGGGAGC
AGC
|
| Construct AA sequence |
ALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPDLQITDQVSL
LRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSD
ACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGS
S
|
| Vector |
p28a-thrombin-lic |
| Construct #12 |
| Construct DNA sequence |
GCACTCACCAACGGGGACCCCCTCAACGGCCACTGCTACCTGTCCGGCTACATCTCGCTGCTGCTGCGCGCCGAGCCCTA
CCCCACGTCGCGCTACGGCAGCCAGTGCATGCAGCCCAACAACATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGC
GCCTGCTCTTCAGCGCCGTCGAGTGGGCCCGCAACATCCCCTTCTTCCCGGATCTGCAGATCACCGACCAGGTGTCCCTG
CTACGCCTCACCTGGAGCGAGCTGTTCGTGCTCAACGCGGCCCAGTGCTCTATGCCGCTGCACGTGGCGCCGTTGCTGGC
CGCCGCCGGCCTGCATGCCTCGCCCATGTCTGCCGACCGCGTCGTGGCCTTCATGGACCACATCCGCATCTTCCAGGAGC
AGGTGGAGAAGCTCAAGGCGCTACACGTCGACTCAGCCGAGTACAGCTGCCTCAAAGCCATCGTGCTGTTCACGTCAGAC
GCCTGTGGCCTGTCGGATGCGGCCCACATCGAGAGCCTGCAGGAGAAGTCGCAGTGCGCACTGGAGGAGTACGTGAGGAG
CCAGTACCCCAACCAGCCCAGCCGTTTTGGCAAACTGCTGCTGCGACTGCCCTCGCTGCGCACCGTGTCCTCCTCCGTCA
TCGAGCAGCTCTTCTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACTCTCATCCGCGATATGTTACTGTCTGGGAGC
AGCTTCAACTGGCCTTACATGTCCATCCAGTGCTCC
|
| Construct AA sequence |
ALTNGDPLNGHCYLSGYISLLLRAEPYPTSRYGSQCMQPNNIMGIENICELAARLLFSAVEWARNIPFFPDLQITDQVSL
LRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSD
ACGLSDAAHIESLQEKSQCALEEYVRSQYPNQPSRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGS
SFNWPYMSIQCS
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-CodonPlus-RIL
Expression outcome: Insoluble
|