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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
NDST4 |
| Description |
N-deacetylase-N-sulfotransferase 4 |
| Nucleotide accession |
NM_022569 |
| Protein accession |
NP_072091 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ACTGACATCAAAATTCTACCATATAGGTCAATGGAGCTGAAAACAGTTAAACCTATTGACACATCCAAAACGGACCCTAC
TGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAGTCCAGCCGATTTCAGT
ACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATT
TATGAAAATATTCTGAAGTATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGT
TAGTATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCA
ATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAA
GGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACA
GACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAA
TTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCA
GGGAAGAGGCTGACATTGTCCTTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAG
GATGAATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACC
TTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGAT
GAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAGCAGAT
GATTCTCAACAAGGAATTTGCACTGGAACATGGAATACCAATCAACATGGGCTATGCTGTGGCCCCACATCACTCAGGGG
TCTACCCGGTTCACATTCAGCTGTATGCAGCTTGGAAGAAGGTCTGGGGTATTCAAGTCACCAGCACT
|
| Construct AA sequence |
TDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVI
YENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEK
GPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLS
GKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVD
EFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTST
|
| Vector |
p28a-thrombin-lic |
| Construct #2 |
| Construct DNA sequence |
CCTACTGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAGTCCAGCCGATT
TCAGTACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAG
TTATTTATGAAAATATTCTGAAGTATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATAC
AGTGTTAGTATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCT
TTTCAATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTG
AGAAAGGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAG
TTACAGACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGA
TGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCT
TGTCAGGGAAGAGGCTGACATTGTCCTTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGA
ACAAGGATGAATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTT
CAACCTTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTG
TGGATGAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAG
CAGATGATTCTCAACAAGGAATTTGCACTGGAACATGGAATACCAATCAACATGGGCTATGCTGTGGCCCCACATCACTC
AGGGGTCTACCCGGTTCACATTCAGCTGTATGCAGCTTGGAAGAAGGTCTGGGGTATTCAAGTCACCAGCACT
|
| Construct AA sequence |
PTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEY
SVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTE
LQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEG
TRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVE
QMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTST
|
| Vector |
p28a-thrombin-lic |
| Construct #3 |
| Construct DNA sequence |
GATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAGTATGTCAGCAT
GGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGTATAATCGGTTTTCATAAAGCCAATG
AGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCAATAATCTAGCTCTAAAGGACTGTTTTGTT
AACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAAGGCCCTCTTCCTGGGGAAGACTGGACTAT
TTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACAGACAGAAAAATCCCTGTCATCCTTGTCTA
GCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTG
AACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCCTTGGACAG
GTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATGAATGTCAAAGATGTGAAGGCATTAC
TAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGGAAGTTTTACCACACA
GGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGATGAGTTCTGGTGGTTTCCTCACATGTGGAG
CCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAGCAGATGATTCTCAACAAGGAATTTGCACTGGAAC
ATGGAATACCAATCAACATGGGCTATGCTGTGGCCCCACATCACTCAGGGGTCTACCCGGTTCACATTCAGCTGTATGCA
GCTTGGAAGAAGGTCTGGGGTATTCAAGTCACCAGCACT
|
| Construct AA sequence |
DIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFV
NPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNL
NFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHT
GTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYA
AWKKVWGIQVTST
|
| Vector |
p28a-thrombin-lic |
| Construct #4 |
| Construct DNA sequence |
ACTGACATCAAAATTCTACCATATAGGTCAATGGAGCTGAAAACAGTTAAACCTATTGACACATCCAAAACGGACCCTAC
TGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAGTCCAGCCGATTTCAGT
ACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATT
TATGAAAATATTCTGAAGTATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGT
TAGTATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCA
ATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAA
GGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACA
GACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAA
TTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCA
GGGAAGAGGCTGACATTGTCCTTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAG
GATGAATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACC
TTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGAT
GAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAGCAGAT
GATTCTCAACAAGGAATTTGCACTGGAACATGGA
|
| Construct AA sequence |
TDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVI
YENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEK
GPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLS
GKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVD
EFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHG
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| Vector |
p28a-thrombin-lic |
| Construct #5 |
| Construct DNA sequence |
CCTACTGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAGTCCAGCCGATT
TCAGTACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAG
TTATTTATGAAAATATTCTGAAGTATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATAC
AGTGTTAGTATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCT
TTTCAATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTG
AGAAAGGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAG
TTACAGACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGA
TGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCT
TGTCAGGGAAGAGGCTGACATTGTCCTTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGA
ACAAGGATGAATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTT
CAACCTTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTG
TGGATGAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAG
CAGATGATTCTCAACAAGGAATTTGCACTGGAACATGGA
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| Construct AA sequence |
PTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEY
SVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTE
LQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEG
TRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVE
QMILNKEFALEHG
|
| Vector |
p28a-thrombin-lic |
| Construct #6 |
| Construct DNA sequence |
GATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAGTATGTCAGCAT
GGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGTATAATCGGTTTTCATAAAGCCAATG
AGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCAATAATCTAGCTCTAAAGGACTGTTTTGTT
AACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAAGGCCCTCTTCCTGGGGAAGACTGGACTAT
TTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACAGACAGAAAAATCCCTGTCATCCTTGTCTA
GCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTG
AACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCCTTGGACAG
GTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATGAATGTCAAAGATGTGAAGGCATTAC
TAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGGAAGTTTTACCACACA
GGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGATGAGTTCTGGTGGTTTCCTCACATGTGGAG
CCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAGCAGATGATTCTCAACAAGGAATTTGCACTGGAAC
ATGGA
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| Construct AA sequence |
DIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFV
NPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNL
NFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHT
GTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHG
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| Vector |
p28a-thrombin-lic |
| Construct #7 |
| Construct DNA sequence |
ACTGACATCAAAATTCTACCATATAGGTCAATGGAGCTGAAAACAGTTAAACCTATTGACACATCCAAAACGGACCCTAC
TGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAGTCCAGCCGATTTCAGT
ACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATT
TATGAAAATATTCTGAAGTATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGT
TAGTATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCA
ATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAA
GGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACA
GACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAA
TTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCA
GGGAAGAGGCTGACATTGTCCTTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAG
GATGAATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACC
TTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGAT
GAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCAC
|
| Construct AA sequence |
TDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVI
YENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEK
GPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLS
GKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVD
EFWWFPHMWSHMQPH
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| Vector |
p28a-thrombin-lic |
| Construct #8 |
| Construct DNA sequence |
CCTACTGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAGTCCAGCCGATT
TCAGTACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAG
TTATTTATGAAAATATTCTGAAGTATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATAC
AGTGTTAGTATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCT
TTTCAATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTG
AGAAAGGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAG
TTACAGACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGA
TGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCT
TGTCAGGGAAGAGGCTGACATTGTCCTTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGA
ACAAGGATGAATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTT
CAACCTTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTG
TGGATGAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCAC
|
| Construct AA sequence |
PTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEY
SVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTE
LQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEG
TRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPH
|
| Vector |
p28a-thrombin-lic |
| Construct #9 |
| Construct DNA sequence |
GATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAGTATGTCAGCAT
GGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGTATAATCGGTTTTCATAAAGCCAATG
AGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCAATAATCTAGCTCTAAAGGACTGTTTTGTT
AACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAAGGCCCTCTTCCTGGGGAAGACTGGACTAT
TTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACAGACAGAAAAATCCCTGTCATCCTTGTCTA
GCAAAACACTCTTTGCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTG
AACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCCTTGGACAG
GTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATGAATGTCAAAGATGTGAAGGCATTAC
TAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGGAAGTTTTACCACACA
GGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGATGAGTTCTGGTGGTTTCCTCACATGTGGAG
CCACATGCAGCCCCAC
|
| Construct AA sequence |
DIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFV
NPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNL
NFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHT
GTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPH
|
| Vector |
p28a-thrombin-lic |
| Construct #10 |
| Construct DNA sequence |
ACCAACCTGAAATTGCAAACTCTGCCTCCAGTGCAACTGGCCCACCAGTATTTTGAGCTCTTCCCTGAGCAGAAAGACCC
TCTATGGCAGAATCCATGTGATGATAAACGACACAAAGACATCTGGTCCAGAGAGAAAACTTGTGACCACTTACCAAAAT
TTCTAGTAATTGGTCCACAAAAAACAGGAACAACTGCACTTTATTTATTTCTTCTTATGCATCCTTCAATCATCAGCAAT
CTCCCTAGTCCAAAGACATTTGAGGAAGTTCAGTTCTTTAATGGCAACAACTATCACAAAGGAATAGACTGGTATATGGA
CTTTTTCCCTACACCATCCAATACTACAAGTGACTTTCTGTTTGAAAAGAGTGCTAATTACTTCCATTCGGAAGAAGCTC
CAAGACGAGCCGCATCTCTTGTCCCCAAAGCCAAGATCATCACCATCCTCATTGACCCCTCAGACAGGGCATACTCTTGG
TACCAGCACCAACGATCACATGAAGATCCAGCTGCTCTGAGGTTCAATTTCTATGAAGTTATTTCAACAGGACATTGGGC
TCCATCTGACTTAAAAACTTTGCAGAGAAGATGCCTAGTACCTGGATGGTATGCAGTCCACATAGAAAGATGGCTAACTT
ACTTTGCTACTTCTCAGTTGCTAATTATTGATGGACAGCAGCTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAG
AAGTTTCTGGGAGTTACACCTCGTTATAATTACTCTGAAGCACTAACGTTTGATCCCCAAAAGGGTTTTTGGTGTCAATT
ACTGGAAGGAGGAAAGACAAAATGCCTTGGAAAAAGCAAAGGCCGAAAATATCCACCTATGGATCCAGAGTCTAGAACGT
TCCTCTCTAATTACTACCGAGATCATAATGTGGAACTATCCAAACTGCTACACAGACTGGGACAGCCTCTGCCATCGTGG
CTGAGACAGGAACTGCAGAAAGTGAGATAG
|
| Construct AA sequence |
TNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISN
LPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSW
YQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQ
KFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSW
LRQELQKVR
|
| Vector |
p28-GST-lic |