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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
NDST2 |
| Description |
N-deacetylase-N-sulfotransferase (heparan glucosaminyl) 2 |
| Nucleotide accession |
NM_003635 |
| Protein accession |
NP_003626 |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
GCTCGAACTGAACCCGTGGTCCTTGTGTTTGTGGAGAGTGCATACTCACAGCTGGGGCAGGAAATTGTGGCCATCCTGGA
GTCTAGTCGTTTTCGTTATAGCACTGAGTTGGCACCTGGCCGAGGGGACATGCCCACATTGACTGATAATACCCATGGCC
GCTATGTCTTGGTCATTTATGAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGACCGGTAC
TGCGTGGAGTATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTT
TCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCC
CCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTG
CTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACA
GGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCG
TTGATGCTGTTGCATACCTCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATC
TTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTT
AGTTCCCAACTTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACG
ACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAAT
CGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGC
TGTGGCCCCCCACCACTCGGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGG
TGACCAGCACTGAGGAGTATCCCCATCTCCGCCCTGCCCGCTACCGCCGTGGCTTCATTCACAATGGCATTATGGTGCTG
CCCCGGCAGACATGTGGCCTCTTCACTCACACAATCTTCTATAATGAGTATCCTGGAGGCTCTCGTGAACTAGACCGGAG
CATCCGAGGTGGAGAGCTCTTTCTGACAGTGCTGCTTAATCCGATCAGCATCTTTATGACCCATCTGTCCAATTATGGAA
ATGACCGGCTGGGCCTATACACCTTTGAGAGCTTGGTGCGCTTCCTCCAGTGTTGGACACGGCTGCGCCTACAGACCCTT
CCTCCTGTCCCACTTGCACAGAAGTACTTTGAACTTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAATCCCTGTGATGA
CAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGTCTCCCGAAGTTCCTCATTGTGGGACCCCAGAAAA
CAGGGACTACAGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTAACTAGCAGCTTCCCTAGCCCCAGCACATTTGAG
GAGATTCAGTTCTTCAACAGCCCTAATTACCACAAGGGTATTGACTGGTACATGGATTTCTTCCCTGTTCCTTCCAATGC
CAGCACTGATTTCCTATTTGAAAAAAGTGCCACCTACTTTGACTCTGAAGTTGTACCACGGCGGGGGGCTGCCCTCCTGC
CACGAGCCAAGATCATCACAGTGCTCACCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCATCAGCGAGCCCATGGA
GACCCAGTTGCTCTGAACTATACCTTCTATCAGGTGATTTCAGCCTCCTCCCAGACCCCTCTGGCACTACGCTCCCTGCA
GAACCGCTGTCTTGTCCCTGGCTACTATTCTACCCATCTACAACGCTGGCTGACTTACTACCCCTCTGGACAGTTGCTGA
TTGTGGATGGGCAAGAGCTGCGTACCAACCCAGCAGCCTCAATGGAGAGCATCCAGAAGTTCCTGGGTATCACACCCTTT
CTGAACTACACACGGACCCTCAGGTTTGATGATGATAAGGGATTTTGGTGCCAGGGACTTGAAGGTGGTAAGACTCGCTG
TCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGATATGGACACTGAGTCCCGTCTTTTCCTTACGGATTTTTTCCGGAACC
ATAATTTGGAGTTGTCGAAGCTGCTGAGCCGGCTTGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAACTGCAGCATTCC
AGTCTGGGC
|
| Construct AA sequence |
ARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRY
CVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPV
LLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDI
FVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHN
RSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVL
PRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTL
PPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFE
EIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHG
DPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLGITPF
LNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHS
SLG
|
| Vector |
p28-GST-lic |
| Construct #2 |
| Construct DNA sequence |
CTACAGACCCTTCCTCCTGTCCCACTTGCACAGAAGTACTTTGAACTTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAA
TCCCTGTGATGACAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGTCTCCCGAAGTTCCTCATTGTGG
GACCCCAGAAAACAGGGACTACAGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTAACTAGCAGCTTCCCTAGCCCC
AGCACATTTGAGGAGATTCAGTTCTTCAACAGCCCTAATTACCACAAGGGTATTGACTGGTACATGGATTTCTTCCCTGT
TCCTTCCAATGCCAGCACTGATTTCCTATTTGAAAAAAGTGCCACCTACTTTGACTCTGAAGTTGTACCACGGCGGGGGG
CTGCCCTCCTGCCACGAGCCAAGATCATCACAGTGCTCACCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCATCAG
CGAGCCCATGGAGACCCAGTTGCTCTGAACTATACCTTCTATCAGGTGATTTCAGCCTCCTCCCAGACCCCTCTGGCACT
ACGCTCCCTGCAGAACCGCTGTCTTGTCCCTGGCTACTATTCTACCCATCTACAACGCTGGCTGACTTACTACCCCTCTG
GACAGTTGCTGATTGTGGATGGGCAAGAGCTGCGTACCAACCCAGCAGCCTCAATGGAGAGCATCCAGAAGTTCCTGGGT
ATCACACCCTTTCTGAACTACACACGGACCCTCAGGTTTGATGATGATAAGGGATTTTGGTGCCAGGGACTTGAAGGTGG
TAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGATATGGACACTGAGTCCCGTCTTTTCCTTACGGATT
TTTTCCGGAACCATAATTTGGAGTTGTCGAAGCTGCTGAGCCGGCTTGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAA
CTGCAGCATTCCAGTCTGGGC
|
| Construct AA sequence |
LQTLPPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSP
STFEEIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQ
RAHGDPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLG
ITPFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREE
LQHSSLG
|
| Vector |
p28-GST-lic |
| Construct #3 |
| Construct DNA sequence |
ATGCTCCAGTTGTGGAAGGTGGTACGCCCAGCTCGGCAGCTGGAACTGCACCGCCTCATACTGCTGCTGATCGCTTTCAG
CCTGGGCTCCATGGGCTTCCTGGCTTATTATGTGTCCACCAGCCCTAAGGCCAAGGAACCCTTGCCCCTGCCCTTGGGAG
ACTGCAGCAGCGGTGGGGCAGCTGGTCCTGGCCCTGCACGGCCTCCAGTTCCACCTCGGCCCCCCAGGCCTCCAGAGACA
GCTCGAACTGAACCCGTGGTCCTTGTGTTTGTGGAGAGTGCATACTCACAGCTGGGGCAGGAAATTGTGGCCATCCTGGA
GTCTAGTCGTTTTCGTTATAGCACTGAGTTGGCACCTGGCCGAGGGGACATGCCCACATTGACTGATAATACCCATGGCC
GCTATGTCTTGGTCATTTATGAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGACCGGTAC
TGCGTGGAGTATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTT
TCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCC
CCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTG
CTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACA
GGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCG
TTGATGCTGTTGCATACCTCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATC
TTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTT
AGTTCCCAACTTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACG
ACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAAT
CGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGC
TGTGGCCCCCCACCACTCGGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGG
TGACCAGCACTGAGGAGTATCCCCATCTCCGCCCTGCCCGCTACCGCCGTGGCTTCATTCACAATGGCATTATGGTGCTG
CCCCGGCAGACATGTGGCCTCTTCACTCACACAATCTTCTATAATGAGTATCCTGGAGGCTCTCGTGAACTAGACCGGAG
CATCCGAGGTGGAGAGCTCTTTCTGACAGTGCTGCTTAATCCGATCAGCATCTTTATGACCCATCTGTCCAATTATGGAA
ATGACCGGCTGGGCCTATACACCTTTGAGAGCTTGGTGCGCTTCCTCCAGTGTTGGACACGGCTGCGCCTACAGACCCTT
CCTCCTGTCCCACTTGCACAGAAGTACTTTGAACTTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAATCCCTGTGATGA
CAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGTCTCCCGAAGTTCCTCATTGTGGGACCCCAGAAAA
CAGGGACTACAGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTAACTAGCAGCTTCCCTAGCCCCAGCACATTTGAG
GAGATTCAGTTCTTCAACAGCCCTAATTACCACAAGGGTATTGACTGGTACATGGATTTCTTCCCTGTTCCTTCCAATGC
CAGCACTGATTTCCTATTTGAAAAAAGTGCCACCTACTTTGACTCTGAAGTTGTACCACGGCGGGGGGCTGCCCTCCTGC
CACGAGCCAAGATCATCACAGTGCTCACCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCATCAGCGAGCCCATGGA
GACCCAGTTGCTCTGAACTATACCTTCTATCAGGTGATTTCAGCCTCCTCCCAGACCCCTCTGGCACTACGCTCCCTGCA
GAACCGCTGTCTTGTCCCTGGCTACTATTCTACCCATCTACAACGCTGGCTGACTTACTACCCCTCTGGACAGTTGCTGA
TTGTGGATGGGCAAGAGCTGCGTACCAACCCAGCAGCCTCAATGGAGAGCATCCAGAAGTTCCTGGGTATCACACCCTTT
CTGAACTACACACGGACCCTCAGGTTTGATGATGATAAGGGATTTTGGTGCCAGGGACTTGAAGGTGGTAAGACTCGCTG
TCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGATATGGACACTGAGTCCCGTCTTTTCCTTACGGATTTTTTCCGGAACC
ATAATTTGGAGTTGTCGAAGCTGCTGAGCCGGCTTGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAACTGCAGCATTCC
AGTCTGGGC
|
| Construct AA sequence |
MLQLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGGAAGPGPARPPVPPRPPRPPET
ARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRY
CVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPV
LLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDI
FVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHN
RSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVL
PRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTL
PPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFE
EIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHG
DPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLGITPF
LNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHS
SLG
|
| Vector |
p11 |
| Construct #4 |
| Construct DNA sequence |
TCCACCAGCCCTAAGGCCAAGGAACCCTTGCCCCTGCCCTTGGGAGACTGCAGCAGCGGTGGGGCAGCTGGTCCTGGCCC
TGCACGGCCTCCAGTTCCACCTCGGCCCCCCAGGCCTCCAGAGACAGCTCGAACTGAACCCGTGGTCCTTGTGTTTGTGG
AGAGTGCATACTCACAGCTGGGGCAGGAAATTGTGGCCATCCTGGAGTCTAGTCGTTTTCGTTATAGCACTGAGTTGGCA
CCTGGCCGAGGGGACATGCCCACATTGACTGATAATACCCATGGCCGCTATGTCTTGGTCATTTATGAGAACCTGCTCAA
GTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGACCGGTACTGCGTGGAGTATGGTGTGGGCATCATTGGCTTTT
TCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTTTCCCCTTTTTTTACACTCAAACTTGGGGCTCCGG
GACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGA
TGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAG
TGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGATGGCATCCAGCGGGTG
CTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACCTCACTGGCAAGCGCCT
CTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGG
CTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTTCAACTTGGGCTTCTCG
GGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTG
GTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGACCAGATGAGGCTCAACA
AACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGCTGTGGCCCCCCACCACTCGGGTGTGTACCCCATC
CACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGGTGACCAGCACTGAGGAGTATCCCCATCTCCGCCC
TGCCCGCTACCGCCGTGGCTTCATTCACAATGGCATTATGGTGCTGCCCCGGCAGACATGTGGCCTCTTCACTCACACAA
TCTTCTATAATGAGTATCCTGGAGGCTCTCGTGAACTAGACCGGAGCATCCGAGGTGGAGAGCTCTTTCTGACAGTGCTG
CTTAATCCGATCAGCATCTTTATGACCCATCTGTCCAATTATGGAAATGACCGGCTGGGCCTATACACCTTTGAGAGCTT
GGTGCGCTTCCTCCAGTGTTGGACACGGCTGCGCCTACAGACCCTTCCTCCTGTCCCACTTGCACAGAAGTACTTTGAAC
TTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAATCCCTGTGATGACAAGAGGCACAAAGATATCTGGTCCAAGGAGAAA
ACCTGTGATCGTCTCCCGAAGTTCCTCATTGTGGGACCCCAGAAAACAGGGACTACAGCTATTCACTTCTTCCTGAGCCT
GCACCCAGCTGTAACTAGCAGCTTCCCTAGCCCCAGCACATTTGAGGAGATTCAGTTCTTCAACAGCCCTAATTACCACA
AGGGTATTGACTGGTACATGGATTTCTTCCCTGTTCCTTCCAATGCCAGCACTGATTTCCTATTTGAAAAAAGTGCCACC
TACTTTGACTCTGAAGTTGTACCACGGCGGGGGGCTGCCCTCCTGCCACGAGCCAAGATCATCACAGTGCTCACCAACCC
TGCTGACAGGGCCTACTCCTGGTACCAGCATCAGCGAGCCCATGGAGACCCAGTTGCTCTGAACTATACCTTCTATCAGG
TGATTTCAGCCTCCTCCCAGACCCCTCTGGCACTACGCTCCCTGCAGAACCGCTGTCTTGTCCCTGGCTACTATTCTACC
CATCTACAACGCTGGCTGACTTACTACCCCTCTGGACAGTTGCTGATTGTGGATGGGCAAGAGCTGCGTACCAACCCAGC
AGCCTCAATGGAGAGCATCCAGAAGTTCCTGGGTATCACACCCTTTCTGAACTACACACGGACCCTCAGGTTTGATGATG
ATAAGGGATTTTGGTGCCAGGGACTTGAAGGTGGTAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGAT
ATGGACACTGAGTCCCGTCTTTTCCTTACGGATTTTTTCCGGAACCATAATTTGGAGTTGTCGAAGCTGCTGAGCCGGCT
TGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAACTGCAGCATTCCAGTCTGGGC
|
| Construct AA sequence |
STSPKAKEPLPLPLGDCSSGGAAGPGPARPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELA
PGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLR
DYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRV
LFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFS
GKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPI
HTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVL
LNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEK
TCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSAT
YFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYST
HLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPD
MDTESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSLG
|
| Vector |
p11 |
| Construct #5 |
| Construct DNA sequence |
GCTCGAACTGAACCCGTGGTCCTTGTGTTTGTGGAGAGTGCATACTCACAGCTGGGGCAGGAAATTGTGGCCATCCTGGA
GTCTAGTCGTTTTCGTTATAGCACTGAGTTGGCACCTGGCCGAGGGGACATGCCCACATTGACTGATAATACCCATGGCC
GCTATGTCTTGGTCATTTATGAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGACCGGTAC
TGCGTGGAGTATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTT
TCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCC
CCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTG
CTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACA
GGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCG
TTGATGCTGTTGCATACCTCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATC
TTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTT
AGTTCCCAACTTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACG
ACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAAT
CGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGC
TGTGGCCCCCCACCACTCGGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGG
TGACCAGCACTGAGGAGTATCCCCATCTCCGCCCTGCCCGCTACCGCCGTGGCTTCATTCACAATGGCATTATGGTGCTG
CCCCGGCAGACATGTGGCCTCTTCACTCACACAATCTTCTATAATGAGTATCCTGGAGGCTCTCGTGAACTAGACCGGAG
CATCCGAGGTGGAGAGCTCTTTCTGACAGTGCTGCTTAATCCGATCAGCATCTTTATGACCCATCTGTCCAATTATGGAA
ATGACCGGCTGGGCCTATACACCTTTGAGAGCTTGGTGCGCTTCCTCCAGTGTTGGACACGGCTGCGCCTACAGACCCTT
CCTCCTGTCCCACTTGCACAGAAGTACTTTGAACTTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAATCCCTGTGATGA
CAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGTCTCCCGAAGTTCCTCATTGTGGGACCCCAGAAAA
CAGGGACTACAGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTAACTAGCAGCTTCCCTAGCCCCAGCACATTTGAG
GAGATTCAGTTCTTCAACAGCCCTAATTACCACAAGGGTATTGACTGGTACATGGATTTCTTCCCTGTTCCTTCCAATGC
CAGCACTGATTTCCTATTTGAAAAAAGTGCCACCTACTTTGACTCTGAAGTTGTACCACGGCGGGGGGCTGCCCTCCTGC
CACGAGCCAAGATCATCACAGTGCTCACCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCATCAGCGAGCCCATGGA
GACCCAGTTGCTCTGAACTATACCTTCTATCAGGTGATTTCAGCCTCCTCCCAGACCCCTCTGGCACTACGCTCCCTGCA
GAACCGCTGTCTTGTCCCTGGCTACTATTCTACCCATCTACAACGCTGGCTGACTTACTACCCCTCTGGACAGTTGCTGA
TTGTGGATGGGCAAGAGCTGCGTACCAACCCAGCAGCCTCAATGGAGAGCATCCAGAAGTTCCTGGGTATCACACCCTTT
CTGAACTACACACGGACCCTCAGGTTTGATGATGATAAGGGATTTTGGTGCCAGGGACTTGAAGGTGGTAAGACTCGCTG
TCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGATATGGACACTGAGTCCCGTCTTTTCCTTACGGATTTTTTCCGGAACC
ATAATTTGGAGTTGTCGAAGCTGCTGAGCCGGCTTGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAACTGCAGCATTCC
AGTCTGGGC
|
| Construct AA sequence |
ARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRY
CVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPV
LLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDI
FVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHN
RSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVL
PRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTL
PPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFE
EIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHG
DPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLGITPF
LNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHS
SLG
|
| Vector |
p11 |
| Construct #6 |
| Construct DNA sequence |
CTACAGACCCTTCCTCCTGTCCCACTTGCACAGAAGTACTTTGAACTTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAA
TCCCTGTGATGACAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGTCTCCCGAAGTTCCTCATTGTGG
GACCCCAGAAAACAGGGACTACAGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTAACTAGCAGCTTCCCTAGCCCC
AGCACATTTGAGGAGATTCAGTTCTTCAACAGCCCTAATTACCACAAGGGTATTGACTGGTACATGGATTTCTTCCCTGT
TCCTTCCAATGCCAGCACTGATTTCCTATTTGAAAAAAGTGCCACCTACTTTGACTCTGAAGTTGTACCACGGCGGGGGG
CTGCCCTCCTGCCACGAGCCAAGATCATCACAGTGCTCACCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCATCAG
CGAGCCCATGGAGACCCAGTTGCTCTGAACTATACCTTCTATCAGGTGATTTCAGCCTCCTCCCAGACCCCTCTGGCACT
ACGCTCCCTGCAGAACCGCTGTCTTGTCCCTGGCTACTATTCTACCCATCTACAACGCTGGCTGACTTACTACCCCTCTG
GACAGTTGCTGATTGTGGATGGGCAAGAGCTGCGTACCAACCCAGCAGCCTCAATGGAGAGCATCCAGAAGTTCCTGGGT
ATCACACCCTTTCTGAACTACACACGGACCCTCAGGTTTGATGATGATAAGGGATTTTGGTGCCAGGGACTTGAAGGTGG
TAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGATATGGACACTGAGTCCCGTCTTTTCCTTACGGATT
TTTTCCGGAACCATAATTTGGAGTTGTCGAAGCTGCTGAGCCGGCTTGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAA
CTGCAGCATTCCAGTCTGGGC
|
| Construct AA sequence |
LQTLPPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSP
STFEEIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQ
RAHGDPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLG
ITPFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREE
LQHSSLG
|
| Vector |
p11 |
| Construct #7 |
| Construct DNA sequence |
CATGGCCGCTATGTCTTGGTCATTTATGAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGA
CCGGTACTGCGTGGAGTATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCA
AGGGCTTTCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTC
ACACGCCCCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGA
ACCAGTGCTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTG
TGGTACAGGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTT
ATCTTCGTTGATGCTGTTGCATACCTCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGA
TGACATCTTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCA
GGACCTTAGTTCCCAACTTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCA
GGGGACGACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTT
CCACAATCGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGG
GGTATGCTGTGGCCCCCCACCACTCGGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGC
ATCCAGGTGACCAGCACTGAGGAGTATCCCCATCTCCGCCCTGCCCGCTACCGCCGTGGCTTCATTCACAATGGC
|
| Construct AA sequence |
HGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHL
TRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKL
IFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDA
GDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWG
IQVTSTEEYPHLRPARYRRGFIHNG
|
| Vector |
p28a-thrombin-lic |
| Construct #8 |
| Construct DNA sequence |
GGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTTTCCCCTTTTTTTACACTC
AAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAG
GGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGG
CCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGA
TGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACC
TCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGG
ACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTT
CAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACC
GCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGAC
CAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGCTGTGGCCCCCCACCACTC
GGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGGTGACCAGCACTGAGGAGT
ATCCCCATCTCCGCCCTGCCCGCTACCGCCGTGGCTTCATTCACAATGGC
|
| Construct AA sequence |
GIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLR
PAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEG
TRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLAD
QMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNG
|
| Vector |
p28a-thrombin-lic |
| Construct #9 |
| Construct DNA sequence |
AACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAGG
GCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGGC
CAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGAT
GGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACCT
CACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGGA
CCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTTC
AACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACCG
CAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGACC
AGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGCTGTGGCCCCCCACCACTCG
GGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGGTGACCAGCACTGAGGAGTA
TCCCCATCTCCGCCCTGCCCGCTACCGCCGTGGCTTCATTCACAATGGC
|
| Construct AA sequence |
NLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHD
GIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTF
NLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHS
GVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNG
|
| Vector |
p28a-thrombin-lic |
| Construct #10 |
| Construct DNA sequence |
CATGGCCGCTATGTCTTGGTCATTTATGAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGA
CCGGTACTGCGTGGAGTATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCA
AGGGCTTTCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTC
ACACGCCCCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGA
ACCAGTGCTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTG
TGGTACAGGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTT
ATCTTCGTTGATGCTGTTGCATACCTCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGA
TGACATCTTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCA
GGACCTTAGTTCCCAACTTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCA
GGGGACGACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTT
CCACAATCGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGG
GGTATGCTGTGGCCCCCCACCACTCGGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGC
ATCCAGGTGACCAGCACTGAGGAGTATCCC
|
| Construct AA sequence |
HGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHL
TRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKL
IFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDA
GDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWG
IQVTSTEEYP
|
| Vector |
p28a-thrombin-lic |
| Construct #11 |
| Construct DNA sequence |
GGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTTTCCCCTTTTTTTACACTC
AAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAG
GGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGG
CCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGA
TGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACC
TCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGG
ACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTT
CAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACC
GCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGAC
CAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGCTGTGGCCCCCCACCACTC
GGGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGGTGACCAGCACTGAGGAGT
ATCCC
|
| Construct AA sequence |
GIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLR
PAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEG
TRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLAD
QMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYP
|
| Vector |
p28a-thrombin-lic |
| Construct #12 |
| Construct DNA sequence |
AACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAGG
GCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGGC
CAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGAT
GGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACCT
CACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGGA
CCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTTC
AACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACCG
CAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGACC
AGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTGGGGTATGCTGTGGCCCCCCACCACTCG
GGTGTGTACCCCATCCACACGCAGCTCTATGAGGCCTGGAAATCCGTGTGGGGCATCCAGGTGACCAGCACTGAGGAGTA
TCCC
|
| Construct AA sequence |
NLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHD
GIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTF
NLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHS
GVYPIHTQLYEAWKSVWGIQVTSTEEYP
|
| Vector |
p28a-thrombin-lic |
| Construct #13 |
| Construct DNA sequence |
CATGGCCGCTATGTCTTGGTCATTTATGAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGA
CCGGTACTGCGTGGAGTATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCA
AGGGCTTTCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTC
ACACGCCCCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGA
ACCAGTGCTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTG
TGGTACAGGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTT
ATCTTCGTTGATGCTGTTGCATACCTCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGA
TGACATCTTTGTGGGCAAGGAAGGGACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCA
GGACCTTAGTTCCCAACTTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCA
GGGGACGACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTT
CCACAATCGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTG
|
| Construct AA sequence |
HGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHL
TRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKL
IFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDA
GDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDL
|
| Vector |
p28a-thrombin-lic |
| Construct #14 |
| Construct DNA sequence |
GGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTTTCCCCTTTTTTTACACTC
AAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAG
GGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGG
CCAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGA
TGGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACC
TCACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGG
ACCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTT
CAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACC
GCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGAC
CAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTG
|
| Construct AA sequence |
GIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLR
PAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEG
TRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLAD
QMRLNKQFALEHGIPTDL
|
| Vector |
p28a-thrombin-lic |
| Construct #15 |
| Construct DNA sequence |
AACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCATCTCACACGCCCCAGCCGCCTAGAACCAGG
GCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATCATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGGC
CAGCTGAGCCCGCAGTGCCAGGACCAGTTCTTCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGAT
GGCATCCAGCGGGTGCTCTTTGGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACCT
CACTGGCAAGCGCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGGA
CCCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAACTTCACCTTC
AACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACGACATGCTGCTGAAGCACCG
CAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCTGTTCCACAATCGCTCCGTGCTGGCTGACC
AGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATTCCCACGGACCTG
|
| Construct AA sequence |
NLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHD
GIQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTF
NLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDL
|
| Vector |
p28a-thrombin-lic |