 |
SGC Experimental Information Sheet |
|
The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
|
|
| Target ID |
MAL7P1.161 |
| Description |
dynein light chain, putative |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
GAAGAACCTAAATGTGATATTTTATATGAACATATGAATTATGAAAATAAAATAAAATTAATTAACATAGCAAAGGAAAT
ATATATAAATATAAATAATAATAAAATAAATTCTTGGAGAAATGCAACTATAGCTTTAAGAGATAAAATAAAGGAAGTTT
TAGATTTTAACGAAAAAGGTTGGCATATAATAATTGGATCCAAATTTGGATTTTTTTGTACTCATGAAATTTATCATGCT
TTGCATTTTAAATTAGATCATATAGAGTTCCTTATTTTTAAACATGGA
|
| Construct AA sequence |
EEPKCDILYEHMNYENKIKLINIAKEIYININNNKINSWRNATIALRDKIKEVLDFNEKGWHIIIGSKFGFFCTHEIYHA
LHFKLDHIEFLIFKHG
|
| Vector |
p28a-thrombin-lic |
| Expression experiment |
Host: BL21-CodonPlus
Expression outcome: Soluble
Crystallization screens:
Red Wings screen,
SGC-I screen
No crystal |