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SGC Experimental Information Sheet |
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The data presented in this document represent preliminary experimental observations
for a de-prioritized SGC target.
We provide this information as a general service to the research community at large,
with the caveat that the data may not have been subjected to rigorous review.
Queries regarding this document or access to reagents may be addressed to
alex.hodgson@utoronto.ca
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| Target ID |
MAL7P1.150 |
| Description |
cysteine desulfurase, putative |
Expression constructs: |
| Construct #1 |
| Construct DNA sequence |
ATGAAATTTCTTCAAATAATAAAACATCTCAAATTACAAAACAAAAAGAACGCACTCGATAATTTTGTAAATTGTCGTAC
CTATGAACATATCAGTAATATTAACAAATTATTCCTCAATAACTTTAGTTCCACGAAGGAACATTCTGAACATGGTCAGG
TAAAACATGAAAACTTCCTGAACAGTACATTAAAATATGAAGAAAACAGCCAAAATGGGTCAACAAATAATTTAAAGAAT
GGAAAGTACAATATGTATGTTTCTGAAGGAAATGTTAATATAAATGAAGAGAAATATAAAGATAACAATATATCTAGTAA
TAATACTCAATATAATAATAATAGTAGTAATTCTGGTAGTTTGAATGATGAAGGACCACTATGGAAAGAGCACATTGATG
ATGTAGTTAACGAAAATAAAAAAAAAAAAATGAACAGATTTTATTTAGACAGTCAAGCAACCACAATGATTGACCCAAGA
GTATTAGATAAGATGCTACCATATATGACATACATATATGGAAATGCTCATTCAAGAAATCATTTTTTTGGATGGGAATC
AGAAAAGGCTGTCGAGGATGCACGAACAAATCTTTTGAATTTAATAAATGGTAAAAACAATAAGGAAATAATATTTACAT
CAGGGGCAACTGAAAGTAATAATCTTGCTTTGATAGGAATATGTACATATTATAATAAATTGAATAAACAAAAGAATCAT
ATTATAACATCTCAAATTGAACATAAATGTATTTTACAAACATGTCGATTTTTACAAACAAAAGGATTTGAAGTAACCTA
TTTAAAACCAGATACAAATGGTTTAGTAAAATTAGATGATATAAAAAACAGTATTAAAGATAATACTATAATGGCATCAT
TTATATTTGTAAATAATGAAATTGGTGTTATACAAGATATTGAAAATATAGGAAATTTGTGTAAAGAAAAAAATATTTTA
TTTCATACGGATGCTTCACAAGCTGCTGGAAAAGTCCCTATCGATGTACAAAAGATGAATATTGATTTAATGTCCATGTC
TGGACATAAATTATATGGACCTAAAGGTATAGGTGCTTTATATATAAAAAGAAAAAAACCAAATATCAGATTAAATGCCT
TAATCCATGGAGGTGGCCAAGAAAGAGGATTAAGATCAGGAACATTACCAACTCATTTAATTGTTGGTTTTGGCGAAGCT
GCTAAGGTATGTTCTTTAGAAATGAATAGAGATGAGAAAAAAGTTAGATACTTTTTTAATTATGTAAAAGATTATTTAAC
GAAACATTTAGATTATATTGTATTTAACGGATGTCAAATAAATAGATATTATGGAAATATGAATATATCCTTTTTATTTG
TTGAAGGAGAAAGTTTATTAATGTCTTTGAATGAAATAGCTCTAAGCTCGGGTTCAGCATGTACATCAAGTACATTAGAA
CCTAGTTATGTGCTTAGATCTATAGGAATTTCAGAAGATATAGCACATACATCTATTAGAATAGGTTTCAATAGATTTAC
TACTTTTTTTGAGGTACAACAATTGTGTATAAATTTAGTAAAATCGGTCGAAAGATTGAGGAGCATCTCACCTCTATATG
AAATGGAGTTAGAAAAAAAAAATCCCTCGAATGACGACATTCCAAAGTTTATATGGACATAA
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| Construct AA sequence |
MKFLQIIKHLKLQNKKNALDNFVNCRTYEHISNINKLFLNNFSSTKEHSEHGQVKHENFLNSTLKYEENSQNGSTNNLKN
GKYNMYVSEGNVNINEEKYKDNNISSNNTQYNNNSSNSGSLNDEGPLWKEHIDDVVNENKKKKMNRFYLDSQATTMIDPR
VLDKMLPYMTYIYGNAHSRNHFFGWESEKAVEDARTNLLNLINGKNNKEIIFTSGATESNNLALIGICTYYNKLNKQKNH
IITSQIEHKCILQTCRFLQTKGFEVTYLKPDTNGLVKLDDIKNSIKDNTIMASFIFVNNEIGVIQDIENIGNLCKEKNIL
FHTDASQAAGKVPIDVQKMNIDLMSMSGHKLYGPKGIGALYIKRKKPNIRLNALIHGGGQERGLRSGTLPTHLIVGFGEA
AKVCSLEMNRDEKKVRYFFNYVKDYLTKHLDYIVFNGCQINRYYGNMNISFLFVEGESLLMSLNEIALSSGSACTSSTLE
PSYVLRSIGISEDIAHTSIRIGFNRFTTFFEVQQLCINLVKSVERLRSISPLYEMELEKKNPSNDDIPKFIWT
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| Vector |
p28a-thrombin-lic |